TAK1/JNK轴通过促进尼罗河鱼的淋巴细胞激活来参与适应性免疫
Ding Wang1, Yuying Zheng1, Jiansong Zhang1
1State Key Laboratory of Estuarine and Coastal Research, School of Life Sciences, East China Normal University, Shanghai, 200241, China.
Fish & shellfish immunology
|July 5, 2024
概括
转化生长因子β激活激酶1 (TAK1) /c-Jun N-终端激酶 (JNK) 轴调节尼罗河鱼的适应性免疫力. 这种保存途径对于鱼类的淋巴细胞激活和免疫反应至关重要.
科学领域:
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
- 鱼类生物学 鱼类生物学
背景情况:
- 转化生长因子β激活激酶1 (TAK1) /c-Jun N-终端激酶 (JNK) 轴是免疫信号通路的关键调节者.
- 它在早期脊椎动物适应性免疫力中的作用,特别是在teleosts中,仍然在很大程度上未被探索.
研究的目的:
- 研究TAK1/JNK轴在尼罗河鱼 (Oreochromis niloticus) 淋巴细胞介导的适应性免疫反应中的调节功能.
- 为了阐明这种保留信号通路在鱼类适应性免疫中的参与.
主要方法:
- 对OnTAK1和OnJNK的比较序列和结构分析.
- 在免疫组织和淋巴细胞中的mRNA表达分析.
- 在体外淋巴细胞激活试验中,使用博12-米里酸13-乙酸加离子素 (P + I) 或PHA.
- 使用特定的TAK1 (NG25) 和JNK (SP600125) 抑制剂的抑制研究.
- 对T细胞激活标记物的分析 (IFN-γ,CD122,IL-2,CD44).
主要成果:
- 在尼罗河鱼的免疫组织中,OnTAK1和OnJNK被保存并广泛表达.
- 它们的表达和酸化水平在细菌感染和体外淋巴细胞激活后显著增加.
- 抑制OnTAK1会降低OnJNK的酸化,抑制OnJNK会降低T细胞激活标记物.
- 已证明TAK1/JNK轴对PHA诱导的T细胞激活至关重要.
结论:
- 保存的TAK1/JNK轴通过控制淋巴细胞激活,在调节尼罗河鱼的适应性免疫反应方面发挥着重要作用.
- 这项研究增强了对远视动物适应性免疫机制的理解,并提供了对鱼类免疫调节的见解.
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