碳酸缓冲器与混合表面技术柱相结合,改善了强烈尾状脂质的峰值形状
Jenny M Nilsson1, David Balgoma2, Curt Pettersson1
1Department of Medicinal Chemistry, Uppsala Biomedical Centre, Uppsala University, Box 574, 75123 Uppsala, Sweden.
Analytica chimica acta
|July 5, 2024
概括
二碳酸 (ABC) 通过减少峰值尾部和增强信号强度,显著改善了染色学中的脂质检测. 这种新方法允许在单个分析中覆盖更广泛的脂质组,从而推进高通量脂质组学.
科学领域:
- 分析化学 分析化学
- 利皮多米克 (Lipidomics) 是一种消化剂.
- 质谱测量质量谱测量
背景情况:
- 酸 (PAs) 和心脂素 (CLs) 在逆相液色谱 (RPLC) 中的检测能力较差,原因是峰值尾巴.
- 目前用于这些脂质的水友互动液态染色学 (HILIC) 方法阻碍了高通量脂质组学.
- 需要改进RPLC方法,以更广泛地覆盖脂质组.
研究的目的:
- 调查二碳酸 (ABC) 对RPLC中脂质的峰值不对称性和检测能力的影响.
- 在传统的BEH C18和HST-CSH C18列上,将ABC与氨酸盐 (AFO) 进行比较.
- 开发一种单一的染色学方法,以提高脂质组覆盖率.
主要方法:
- 研究了2.5mM的ABC缓冲区 (pH8) 与AFO对峰值形状和检测能力的影响.
- 使用了传统的BEH C18和HST-CSH C18柱.
- 在负模式下使用RPLC-ESI-Q-TOF-MS分析了酸 (PA),心脂素 (CL) 和其他脂素.
主要成果:
- 2.5mM ABC和HST-CSH C18柱的组合使PA 16:0/18:1的不对称系数从8.4降低到1.6.
- 与AFO和BEH C18柱相比,PA 16:0/18:1的[M-H]−离子的峰值高度提高了多达54倍.
- 在HST-CSH C18列中,在负模式下对脂和脂的检测能力提高了 (高达28倍).
- 成功地将该方法应用于小鼠肝脏样本,揭示了以前未被检测到的内源性脂.
结论:
- 使用2.5毫米ABC显著改善了在负模式RPLC-ESI-Q-TOF-MS中对PA和其他脂质的峰值形状和检测能力.
- 带有ABC的HST-CSH C18列在单次注射中提供了增强的脂质组覆盖.
- 这种方法适合未来的高通量脂质学应用.
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