在电膜提取过程中使用的固体支物的化学性质演变:基于米数工具的比较分析
Cristina Román-Hidalgo1, Mercedes Villar-Navarro1, María Jesús Martín-Valero1
1Department of Analytical Chemistry, Faculty of Chemistry, Universidad de Sevilla, C/Prof. García González, s/n, 41012, Seville, Spain.
Analytica chimica acta
|July 5, 2024
概括
绿色分析化学 (GAC) 通过使用可生物降解的酸盐膜推进了电膜提取 (EME). 这些环保支持通过消除有机溶剂来提高分析性能和可持续性.
科学领域:
- 分析化学 分析化学
- 绿色化学 绿色化学
- 材料科学 材料科学 材料科学
背景情况:
- 绿色化学原则推动了环保分析方法的发展.
- 样本准备,一个关键的分析步骤,正在优化可持续性.
- 电膜提取 (EME) 传统上使用塑料支架,但正在向生物聚合物等更绿色的替代品发展.
研究的目的:
- 在绿色分析化学 (GAC) 的框架内,评估EME中不同固体支物的环境影响.
- 评估使用新生物材料的EME程序的可持续性,考虑分析性能和可行性.
- 将生物聚合物基膜的效率和环保性与传统支器进行比较.
主要方法:
- 绿色指标工具的应用:分析生态尺度,复杂GAPI和AGREEprep,以评估EME的固体支持演变.
- 使用RGB模型和BAGI指标来评估EME程序的整体可持续性.
- 研究生物聚合物膜的使用,特别是和奇托,作为传统EME支的替代品.
主要成果:
- 基托桑基支架在EME中展示了最有效和最环保的分析方法.
- 提高的性能归因于奇托固有的特性和有机溶剂的消除.
- 与传统支物相比,生物材料显著减少了EME程序对环境的影响.
结论:
- 生物降解材料,特别是酸盐,为开发EME中的绿色分析方法提供了显著的优势.
- 在EME中使用生物聚合物提高了量化参数和整体程序的可持续性.
- 在EME程序中使用奇托桑可以提高分析性能,减少环境足迹.
相关概念视频
00:56Extraction: Advanced Methods
444
Metal ions can be separated from one another by complexation with organic ligands–the chelating agent– to form uncharged chelates. Here, the chelating agent must contain hydrophobic groups and behave as a weak acid, losing a proton to bind with the metal. Since most organic ligands used in this process are insoluble or undergo oxidation in the aqueous phase, the chelating agent is initially added to the organic phase and extracted into the aqueous phase. The metal-ligand complex is...
444
01:30Capillary Electrophoresis: Applications
373
Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
373
01:09Ion-Exchange Chromatography
442
Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
442
01:18Detergent Purification of Membrane Proteins
5.2K
Detergents are used to purify the integral proteins of the membrane. The hydrophobic portion of the detergent can replace membrane phospholipids while solubilizing the membrane proteins. When detergent monomers reach a specific concentration in a solution called critical micelle concentration (CMC), they form micelles. Above CMC, the concentration of the detergent monomers remains in equilibrium with the micelle. The number of detergent monomers present in the CMC varies for each detergent, and...
5.2K
01:17Ion Exchange
573
Ion exchange chromatography separates charged molecules from a solution by reversibly exchanging them with mobile, or 'active', ions associated with the oppositely charged stationary phase. This method can be used to separate ions, soften and deionize water, and purify solutions. The polymers comprising the ion-exchange column are high-molecular-weight and chemically stable polymers, crosslinked to be porous and essentially insoluble. They are also functionalized with either acidic or...
573
01:20Preparation of Samples for Electron Microscopy
5.4K
To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...
5.4K