在饮料中有效分离有机离子,使用氨基功能化毛细体电泳与非接触式导电检测
Apinya Obma1, Rawiwan Bumrungpuech1, Pattamaporn Hemwech1
1Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Mahidol University, Rama VI Rd., Bangkok, 10400, Thailand.
Analytica chimica acta
|July 5, 2024
概括
使用3-aminopropyltriethoxysilane (APTES) 的永久毛细管涂层提供了通过毛细管电泳 (CE) 进行阳离子分析的稳定和高效方法. 这种方法克服了传统动态涂层的缓慢平衡问题,使离子在饮料等复杂样品中能够准确可靠地分离出来.
科学领域:
- 分析化学 分析化学
- 分离科学 分离科学
背景情况:
- 毛细管电泳 (CE) 能够使用逆电流 (EOF) 进行快速的离子分析.
- 传统的动态涂层 (例如, cetyltrimethylammonium化物) 用于EOF反转,表现出缓慢的平衡和峰值漂移.
- 永久毛细管表面修改提供了一个潜在的解决方案,以提高稳定性和可重复性.
研究的目的:
- 为了研究3-aminopropyltriethoxysilane (APTES) 对永久毛细血管表面修饰的共价结合.
- 评估APTES度对EOF流动性,分离效率和复制性的影响.
- 为了比较APTES功能化的毛细血管与传统动态涂层的性能.
主要方法:
- 用不同度的3-aminopropyltriethoxysilane (APTES) 进行毛囊表面功能化.
- 测量电流 (EOF) 流动性,分离效率和可重复性.
- 使用X射线光电子光谱 (XPS) 和接触角 (CA) 测量进行表征.
- 在饮料样本中分析21离子和有机离子的标准混合物.
主要成果:
- APTES覆盖范围和随后的EOF依赖于度,由XPS和CA测量证实.
- 与CTAB动态涂层相比,APTES功能化的毛细血管显示出更快的平衡和优越的长期EOF稳定性.
- 通过优化APTES涂层,在10分钟内实现了21个离子的基线分离.
- 成功证明了各种饮料中有机离子的可靠和精确分析.
结论:
- 联APTES涂层提供了一个简单而稳定的方法,用于在毛细管电泳中永久逆转EOF,以确定离子.
- APTES的度允许可调节的EOF,使不同的样本复杂性和分离要求能够优化.
- 这种方法提高了分离效率和灵敏度,对于分析饮料中的有机酸有效.
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