子大小与转动物微生物组结构和山羊的代谢概况的关联
Sayed Haidar Abbas Raza1,2,3,4, Muhammad Khan1, Xiaojun Ni1,5,6
1Yunnan Animal Science and Veterinary Institute, Kunming City, Yunnan Province, China.
Scientific reports
|July 5, 2024
概括
云山黑山羊的垃圾大小影响食新陈代谢物和微生物群落,影响山羊的生育能力. 这项研究揭示了与低与高 litter 尺寸相关的特定微生物和代谢变化.
科学领域:
- 动物科学动物科学
- 微生物学 微生物学
- 代谢学 代谢学 代谢学
背景情况:
- 垃圾大小对山羊养殖经济有重大影响.
- 与怀孕相关的因素,包括 litter 尺寸,可能会调节 ruminal microbiome.
- 了解这些关联可以改善山羊育种策略.
研究的目的:
- 为了研究低 litter 尺寸和高 litter 尺寸和动物微生物组结构之间的关联,在云山黑人做.
- 分析与不同子大小相关的代谢概况.
- 探索虫微生物群落与云山黑山羊中的代谢物之间的相关性.
主要方法:
- 16S rRNA基因测序用于小动物微生物群的概况.
- 液体染色学-质谱学 (LC-MS/MC) 用于新陈代谢分析.
- 来自低 (≤2个孩子) 和高 (≥3个孩子) litter 规模组的转液的比较分析.
主要成果:
- 虽然整体的反动物微生物群结构没有显著变化,但在高 litter 规模组中,特定的细菌 (Ruminococcaceae_UCG-009,Sediminispirochaeta,Paraprevotella) 增加.
- 在低 litter 尺寸组和高 litter 尺寸组之间观察到 79 种代谢物的显著差异,在高 litter 组的负离子模式中显著上调.
- 已识别的代谢物涉及至关重要的生化途径,包括蛋白质消化和mTOR信号通路.
- 斯皮尔曼的相关性分析证实了微生物群落和代谢物之间的紧密相关性,在子大小组之间存在差异.
结论:
- 垃圾大小会影响云山黑山羊的体微生物组成和代谢概况.
- 这些发现提供了关于肠微生物群和与山羊生育相关的代谢学调节机制的见解.
- 这项研究为养殖者提供了潜在的启发,以提高云山黑山羊的生育能力.
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