一个单样样本的工作流程用于临床样本的联合代谢和蛋白质组分析
Hagen M Gegner1, Thomas Naake2, Karim Aljakouch3,4
1Centre for Organismal Studies (COS), Metabolomics Core Technology Platform, Heidelberg University, Im Neuenheimer Feld 360, 69120, Heidelberg, Germany.
Clinical proteomics
|July 5, 2024
概括
一个新的工作流,MTBE-SP3,可以从单个样本同时进行蛋白质组和代谢组分析. 这种方法提高了多学科研究的标准化和可扩展性,特别是在有限的样本可用性的情况下.
科学领域:
- 生物化学和分子生物学
- 系统生物学 系统生物学
- 生物技术是生物技术.
背景情况:
- 了解细胞调节需要整合蛋白质组和代谢组数据.
- 现有的多omics工作流通常需要单独的样本处理,增加变化和样本输入.
- 标准化和可扩展性对于大规模的生物和临床研究至关重要.
研究的目的:
- 引入和评估一个统一的工作流 (MTBE-SP3) 从单个生物样本进行并行蛋白质组和代谢组分析.
- 评估MTBE-SP3工作流中的蛋白质基因分析性能,并将其与已确定的方法进行比较.
- 证明工作流在各种生物矩阵中的适用性及其在临床研究中的实用性.
主要方法:
- 将已建立的代谢学 (EtOH/MTBE) 和蛋白质学 (autoSP3) 协议集成到一个单一的工作流中.
- 利用从代谢取中产生的蛋白质残留物作为蛋白质组分析的直接输入.
- 通过各种生物样本进行验证,包括FFPE组织,新鲜冷组织,血,血清和细胞.
主要成果:
- 通过MTBE-SP3获得的蛋白质组概况的证明相当性,无论之前的代谢物提取如何.
- 展示了该工作流对各种生物矩阵的适用性,支持其在临床环境中的应用.
- 将MTBE-SP3应用于肺腺癌队列,透露一致的蛋白质组变化,并通过集成的奥米克数据识别线粒体功能障碍.
结论:
- MTBE-SP3 工作流便于从同一样本中可靠地并行测量蛋白质和代谢物,减少变异和样本输入.
- 这种方法对于样本可用性有限的研究尤其有利,增强了多学科数据的整合.
- MTBE-SP3促进了标准化和可扩展性,为大规模的临床和生物研究提供了巨大的潜力.
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