与M2型瘤相关的巨细胞通过PI3K/AKT通路在宫癌中调高PD-L1表达
Fan Guo1,2, Weina Kong1, Dewei Li3
1Department of Medical Laboratory Center, Tumor Hospital Affiliated to Xinjiang Medical University, State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia, No 789 Suzhou Road, Urumqi, 830011, Xinjiang, China.
European journal of medical research
|July 5, 2024
概括
瘤相关巨细胞 (TAMs),特别是M2型,增强PD-L1表达并促进宫癌的进展. 向TAM可能会改善宫癌治疗中的PD-1/PD-L1抑制剂疗效.
科学领域:
- 免疫学 免疫学 免疫学
- 在瘤学瘤学.
- 分子生物学分子生物学
背景情况:
- 编程细胞死亡蛋白1 (PD-1) /PD-1配体 (PD-L1) 抑制剂在癌症治疗中表现有前途.
- 宫癌 (CC) 对PD-1/PD-L1抑制剂的反应率较低,与免疫抑制性瘤微环境 (TME) 有关.
- 与瘤相关的巨细胞 (TAMs) 导致抑制性TME,并影响治疗结果.
研究的目的:
- 阐明TAMs对宫癌中PD-L1表达的调节机制.
- 在CC患者中调查TAM透和PD-L1表达之间的相关性.
- 探索TAMs对CC细胞行为和进展的影响.
主要方法:
- 免疫组织化学测试以评估CC组织中PD-L1,CD68和CD163的表达.
- 生物信息学分析以确定参与PD-L1调节的巨亚型.
- 共同培养模型评估TAM对CC细胞形态,迁移和入侵以及PD-L1调节的影响.
主要成果:
- 在瘤细胞上的PD-L1表达与预后不佳和CD163+TAM透相关.
- 生物信息学分析表明PD-L1表达和M1/M2TAM透之间存在关联.
- 共同培养实验显示,M1/M2 TAMs上调PD-L1,M2 TAMs可能通过PI3K/AKT通路起作用. 此外,TAM还增强了CC细胞的迁移和入侵.
结论:
- PD-L1表达作为CC的预后因素,与CD163+TAM透有关.
- M2 TAMs通过PI3K/AKT通路对CC细胞中的PD-L1表达进行上调,促进瘤进展.
- TAMs影响CC细胞迁移,入侵和整体瘤进展,表明治疗向潜力.
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