相关实验视频

Updated: Jun 21, 2025

A Rabbit Venous Interposition Model Mimicking Revascularization Surgery using Vein Grafts to Assess Intimal Hyperplasia under Arterial Blood Pressure

A Rabbit Venous Interposition Model Mimicking Revascularization Surgery using Vein Grafts to Assess Intimal Hyperplasia under Arterial Blood Pressure

Published on: May 15, 2020

库库比他素通过抑制环素A2的表达和抑制VSMC的增殖来缓解血管新极度增生症

Ruqiang Yuan¹, Lei Qian¹, Hu Xu²

¹Advanced Institute for Medical Sciences, Dalian Medical University, Dalian, China.

Animal models and experimental medicine

|July 6, 2024

概括

来自Cucumis melo L. (CuECs) 的甲酸,特别是甲酸B (CuB),抑制了血管光滑肌肉细胞的增殖和新极度增生,从而有可能预防复原症. 这些化合物阻断细胞循环并降低环林A2的表达.

科学领域:

药理学药理学是指药理学的学科.
心血管生物学心血管生物学
自然产品化学化学

背景情况:

血管造形术后的复原症是一个重大的临床挑战.
释放药物的支架旨在通过抑制血管光滑肌细胞 (VSMC) 增殖来预防复原.
甲酸显示出抗增殖和低血压的作用.

研究的目的:

调查来自Cucumis melo L. (CuECs) 的库库比他和库库比他B (CuB) 对的作用.
评估CuECs和CuB在VSMC上的抗扩散潜力.

主要方法:

在体内研究使用C57BL/6小鼠与动脉绑定,治疗CuECs或CuB.
组织学分析 (H&E,免疫光,免疫组织化学) 针对新极端增生症.
细胞增殖和细胞循环分析的体外试验 (西斑,qPCR,流细胞计,EDU,免疫光学).
分子对接以评估与环林A2.2的相互作用.

主要成果:

CuECs和CuB显著抑制了新极端增生和VSMC增殖.
这两种化合物都在S阶段诱导细胞循环停止.
分子对接表明库库比塔辛对林A2具有很高的结合亲和力.
在体外和体内,CuECs和CuB降低了环素A2的表达.

结论:

CuECs,特别是CuB,对VSMCs表现出显著的抗增殖作用.
这些发现表明CuECs和CuB是预防复原的有前途的治疗药物.

关键词:

这就是为什么CuBB是CuB.这种子叫做"子".环 A2 环 A2 环 A2 是一种静止性静止症是一种静止症.血管光滑肌肉细胞细胞

更多相关视频

Vascular Balloon Injury and Intraluminal Administration in Rat Carotid Artery

Vascular Balloon Injury and Intraluminal Administration in Rat Carotid Artery

Published on: December 23, 2014

A Rat Carotid Artery Pressure-Controlled Segmental Balloon Injury with Periadventitial Therapeutic Application

A Rat Carotid Artery Pressure-Controlled Segmental Balloon Injury with Periadventitial Therapeutic Application

Published on: July 9, 2020

相关概念视频

Inhibition of Cdk Activity

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...

Anaphase Promoting Complex

Anaphase Promoting Complex

The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...

Regulation of Angiogenesis and Blood Supply

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...

Antihypertensive Drugs: Vasodilators

Antihypertensive Drugs: Vasodilators

Vasodilators, primarily affecting the smooth muscles within arterial and venous walls, are commonly used for hypertension treatment. Medications such as minoxidil and hydralazine primarily target arteries and arterioles, while sodium nitroprusside acts on arterioles and venules. Minoxidil, functioning as a prodrug, is metabolized by hepatic sulfotransferase into its active form, minoxidil sulfate, after oral administration. This metabolite binds to the sulfonylurea receptor (SUR) component of...

Drugs that Destabilize Microtubules

Drugs that Destabilize Microtubules

Microtubules are dynamic structures and can be regulated by microtubule targeting agents (MTAs). Microtubule destabilizing drugs are a class of MTAs that destabilize and prevent microtubules' polymerization. Both natural and synthetic chemicals can be found under this class of drugs. Vincristine and vinblastine, two vinca alkaloids, and colchicine were among the first to be discovered. These drugs can affect cells in various ways, either by inducing a change in cell morphology, preventing...

Mechanism of Angiogenesis

Mechanism of Angiogenesis

Blood vessel formation starts early during embryonic development, around day 7. In the extraembryonic yolk sac, mesodermal precursor cells called hemangioblast proliferate and differentiate into angioblast. Angioblasts express vascular endothelial growth factor receptor 2 or VEGFR2, which binds VEGF-A, a proangiogenic factor, guiding blood vessel formation. VEGF signaling promotes angioblasts to form a blood island in the developing embryo. Angioblasts further differentiate, giving rise to...