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在极端耐受性或极端友好性真菌中形成孔隙的艾杰罗素和MACPF蛋白质

Nada Kraševec¹

¹Department of Molecular Biology and Nanobiotechnology, National Institute of Chemistry, Ljubljana, Slovenia.

|July 6, 2024

概括

艾格罗利辛和膜攻击复合体/氨酸类蛋白质 (MACPF) 是形成孔隙的蛋白质. 这些蛋白质很少在极端的真菌中发现,这表明它们在一般性真菌的竞争性排斥中起作用.

科学领域:

菌类学菌类学是指菌类学.
分子生物学分子生物学
生态生态学生态生态学

背景情况:

艾格罗林蛋白与分子受体相互作用,可能与膜攻击复合物/蛋白 (MACPFs) 等合作伙伴形成毛孔.
适应极端环境的真菌包括一般主义者和专家,专家由于竞争对手较少而产生更简单的分子.
艾格罗利辛在真菌基因组中分布不均,这促使人们对其在极端生存中的作用进行了调查.

研究的目的:

调查艾杰罗利辛及其伴侣蛋白是否有助于通用和专业真菌的极端生存策略.
在多种极端耐受性和极端友性真菌中分析艾杰罗利辛和MACPF的存在.

主要方法:

编制了109种耐受各种极端条件 (温度,pH,盐度,金属) 的真菌物种列表.
解决了包括真菌物种命名,定义极端耐受性特征和将分离物与基因组联系在内的挑战.
分析了基因组数据,以确定在选定的真菌物种中存在eigerolysin和MACPF基因.

主要成果:

与所有真菌基因组相比,在极端耐受性/极端友好性真菌中,编码ejerolysins或MACPF的基因组的频率较低.
在极端菌基因组中没有发现孔形成基因对的候选者.
在只有两种物种中确定了Aegerolysin和MACPF的合作伙伴对:Apergillus niger和A. foetidus,这两种都是无处不在的金属耐受性通用主义者.

结论:

这些发现支持这样的假设,即艾杰罗素-MACPF对导致真菌的竞争排斥.
极端菌中这些孔形成蛋白质的有限存在表明这些生物体中存在替代生存机制.

关键词:

艾格罗里辛斯 (Aegerolysins) 是一种在地球上的生物.竞争中的排斥竞争中的排斥极端爱好者的极端爱好者极端耐受性极端耐受性菌是一种真菌.膜攻击复合物/氨酸 (MACPF) 领域形成孔隙的蛋白质.

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