劳伦斯菌 (Cryptococcus laurentii):一种用于从农业副产品中产生西兰酶的野生酵母
Deborah Murowaniecki Otero1,2, Bruno Perret3, Liliane Teixeira3
1Graduate Program in Food, Nutrition, and Health, Nutrition School, Federal University of Bahia, 32 Araújo Pinho, Salvador, Brazil.
概括
农业工业的副产品,如脱脂的米,可以在成本效益的基础上用于生产内分离酶. 这种酵母驱动的生物工艺为酶生产提供了一种可持续的方法,降低了成本和环境影响.
科学领域:
- 生物技术是生物技术.
- 酶的生产 酶的生产
- 微生物发酵 微生物发酵
背景情况:
- 以竞争性成本生产酶对生物技术至关重要.
- 农业工业副产品为酶生产提供了一种可持续且具有成本效益的替代品.
- 利用废物材料将环境影响降至最低.
研究的目的:
- 使用富含西兰的农业工业副产品生产内-西兰酶.
- 为了评估不同基纤维素材料作为酵母酵母生产的基质的有效性.
- 为了确定成本效益高的酶合成的最佳条件.
主要方法:
- 培养酵母Cryptococcus laurentii使用五种纤维素材料 (脱脂大米,大米,玉米,麦,大豆).
- 材料先用NaOH (4%w/v) 进行预处理,并以2% (w/v) 添加到培养基中.
- 在激发培养96小时后测量了酶活性.
主要成果:
- 脱脂的米产生了最高的内分氧化酶活性 (pH 7.3 的8.7 U/mL).
- 玉米产生的酶产量最低 (1.6 U/mL).
- 预处理和特定的副产品成分显著影响了酶产量.
结论:
- 脱脂的米是Cryptococcus laurentii产生的内分氧化酶的一个非常适合的基质.
- 这项研究表明,利用农业工业废弃物实现高效,可持续的酶制造的潜力.
- 对优化大米利用条件的进一步研究可以增强生物技术应用.
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