在Bulang茶发酵过程中微生物多样性和挥发性代谢物的变化
Jinping Zhou1, Laifeng Chen1, Hooi Ling Foo2
1College of Food Science and Technology, Yunnan Agricultural University, Heilongtan, Kunming 650201, People's Republic of China.
Food chemistry
|July 6, 2024
概括
这项研究揭示了布朗茶发酵中的微生物群落,短链脂肪酸 (SCFA) 和挥发性化合物. 植物乳杆菌和Cladosporium sphaerospermum显著影响了风味的发展.
科学领域:
- 食品科学 食品科学 食品科学
- 微生物学 微生物学
- 代谢学 代谢学 代谢学
背景情况:
- 布朗茶是一种传统的发酵产品,具有独特的风味特征.
- 了解其发酵过程对于质量控制和风味增强至关重要.
研究的目的:
- 为了研究在布朗茶发酵过程中的微生物社区结构.
- 分析短链脂肪酸 (SCFA) 和挥发性化合物的变化.
- 阐明微生物,SCFA和挥发性化合物在风味形成中的关系.
主要方法:
- 16S rRNA和ITS基因测序用于微生物社区分析.
- 气色谱-质谱 (GC-MS) 用于挥发性化合物分析.
- 斯皮尔曼相关性分析将微生物,SCFA和挥发性数据联系起来.
主要成果:
- 微生物群体主要由植物性乳杆菌,未分类的肠杆菌,未分类的Debaryomyces,Candida metapsilosis,Cladosporium sphaerospermum和未分类的Aspergillus.
- 在发酵过程中,SCFA总量增加,酸是最丰富的.
- 确定了398种不同的挥发性代谢物,根据气味活性值 (OAV) 确定了10种关键化合物.
- 植物乳杆菌和Cladosporium sphaerospermum与SCFA和挥发性化合物有很强的相关性.
结论:
- 微生物活动,特别是L. plantarum和C. sphaerospermum,在布朗茶中SCFA和挥发性化合物的形成中发挥着重要作用.
- 这项研究为了解布朗茶的风味发展机制提供了理论基础.
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