使用流式细胞计量对高压处理的Bacillus subtilis子种群进行表征 - - 阐明子在550MPa时的超休眠状态
Rosa Heydenreich1, Alessia I Delbrück1, Christina Peternell1
1Sustainable Food Processing Laboratory, Institute of Food, Nutrition, and Health, Department of Health Science and Technology, ETH Zurich, Zurich, Switzerland.
International journal of food microbiology
|July 6, 2024
概括
这项研究描述了超休眠的细菌子,确定了短暂的因素,而不是永久的变化,是它们对非常高压 (vHP) 失活的抵抗的原因. 了解这一点可以改善食品保存策略.
科学领域:
- 微生物学 微生物学
- 食品科学 食品科学 食品科学
- 生物技术是生物技术.
背景情况:
- 细菌子耐药性对工业无活化提出了挑战.
- 高压 (HP) 可以诱导子发芽,有助于无活化.
- 不同质的发芽导致持久的,耐药的超休子.
研究的目的:
- 为了表征非常高压 (vHP) 超休眠的 Bacillus subtilis 子.
- 了解vHP处理的子中发芽缺陷的原因.
- 为了验证流细胞计 (FCM) 量化超休子.
主要方法:
- 细菌细菌子受到550MPa和60°C的非常高压 (vHP) 的影响.
- 使用化 (PI) 和SYTO16的流细胞计 (FCM) 已被验证用于量化未发芽的子.
- 单独的超休子被分析为二皮科林酸含量和随后的发芽能力.
主要成果:
- 流细胞计 (FCM) 在短时间的vHP治疗和潜伏后有效量化了超休子.
- 超休眠子显示出类似的二皮可林酸含量和后代的正常发芽.
- 在第二次vHP治疗中,孤立的超休子表现出增强的发芽,这表明vHP超休的暂时原因.
结论:
- 乙型HP超休症很可能是由短暂的因素引起的,而不是永久的遗传或组成变化.
- 了解vHP超休眠对于开发食品加工中有效的子无活化策略至关重要.
- 这项研究为改善基于轻度HP的食品保存方法提供了基本的见解.
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