Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

4.0K
Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.
4.0K
Photoluminescence: Applications01:14

Photoluminescence: Applications

386
Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
386

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Tutorial: biomembranes in hybrid living bioelectronics.

Nature protocols·2026
Same author

Multicentre validation and update of a Legionella prediction score to guide microbiological testing in community-acquired pneumonia.

International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases·2026
Same author

Diagnostic performance and interchangeability of two commercial anti-RSV-IgG and IgA ELISAs across clinically relevant populations.

Microbiology spectrum·2026
Same author

Rare twin cysteine residues in the HIV-1 envelope variable region 1 link to neutralization escape and breadth development.

Cell host & microbe·2026
Same author

Low-positive CMV IgG serostatus before allogeneic hematopoietic cell transplantation is not associated with increased CMV reactivation risk.

Bone marrow transplantation·2026
Same author

Detecting <i>erm</i>-Mediated Inducible Macrolide-Lincosamide-Streptogramin B Resistance in Anaerobic Clinical Isolates.

Antibiotics (Basel, Switzerland)·2026

相关实验视频

Updated: Jun 21, 2025

Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds
10:10

Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds

Published on: November 13, 2021

7.9K

基于的电化学金量化方法用于横流量测试.

Yves Blickenstorfer1, Vlastimil Jirasko1, Alexander Tanno1

  • 1Laboratory of Biosensors and Bioelectronics, Institute of Biomedical Engineering, ETH Zurich, Zurich, Switzerland; Hemetron Ag, Thalwil, Switzerland.

Biosensors & bioelectronics
|July 6, 2024
PubMed
概括

我们开发了一种新的电化学方法,用于横向流量测试 (LFA),为家庭诊断提供定量和敏感的结果. 这一突破改进了传统的对/否测试,提供了更高的准确性和更低的检测极限.

更多相关视频

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays
07:13

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays

Published on: June 28, 2024

1.2K
Fabricating a UV-Vis and Raman Spectroscopy Immunoassay Platform
09:02

Fabricating a UV-Vis and Raman Spectroscopy Immunoassay Platform

Published on: November 10, 2016

10.4K

相关实验视频

Last Updated: Jun 21, 2025

Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds
10:10

Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds

Published on: November 13, 2021

7.9K
Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays
07:13

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays

Published on: June 28, 2024

1.2K
Fabricating a UV-Vis and Raman Spectroscopy Immunoassay Platform
09:02

Fabricating a UV-Vis and Raman Spectroscopy Immunoassay Platform

Published on: November 10, 2016

10.4K

科学领域:

  • 生物医学工程 生物医学工程
  • 分析化学 分析化学
  • 临床诊断 临床诊断 临床诊断 临床诊断

背景情况:

  • 侧流检测 (LFA) 提供了方便,经济高效,快速的家庭医疗诊断.
  • 目前的LFA仅限于定性 (是/否) 结果,缺乏实验室方法的灵敏度.
  • 目前用于定量LFA的光学阅读器通常太昂贵,无法在家中广泛使用.

研究的目的:

  • 为LFA开发一种定量和灵敏的读取方法.
  • 克服当前高质量的LFA和昂贵的光学阅读器的局限性.
  • 为了在家中实现更先进的医疗诊断.

主要方法:

  • 为LFA开发了一种电化学读取程序.
  • 使用电压触发的黄金纳米粒子现场溶解 (传统的LFA标签).
  • 通过电和电化学测量量量化溶解黄金,实现低噪音电流信号.

主要成果:

  • 与传统光学技术相比,实现了更高的检测极限.
  • 证明了LFA的定量和敏感的电化学读数.
  • 为读出系统开发了硬件,显示了低成本电子产品的潜力.

结论:

  • 新的电化学读数显著提高了LFA在家诊断的能力.
  • 这种方法提供了更好的灵敏度和定量结果,超越了光学限制.
  • 该技术可用于低成本的电子设备,为可访问的先进诊断铺平了道路.