PRRSV NSP1α通过蛋白酶体系统降解TRIM25,以抑制宿主抗病毒免疫反应

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Yuhang Zheng¹, Dandan Jiang², Chao Sui³

¹College of Veterinary Medicine, Qingdao Agricultural University, Qingdao 266109, China.

Veterinary microbiology

|July 6, 2024

概括

猪生殖和呼吸系统综合征病毒 (PRRSV) NSP1α蛋白降解TRIM25,这是一个关键的抗病毒蛋白. 这种降解抑制了宿主.

科学领域:

病毒学病毒学
免疫学免疫学免疫学
分子生物学分子生物学

背景情况:

猪生殖和呼吸系统综合征 (PRRS) 是猪生产中的一个主要经济威胁,由PRRSV引起.
I型干扰素 (IFN) 和干扰素刺激基因 (ISG) 对于抑制PRRSV感染至关重要.
PRRSV采用策略来抵消宿主的先天免疫反应,包括干扰IFN生产.

研究的目的:

研究除了N蛋白之外,其他PRRSV蛋白是否可以抑制TRIM25的抗病毒功能.
阐明PRRSV蛋白可能对抗TRIM25的抗病毒活性的机制.

主要方法:

评估了PRRSV NSP1α对TRIM25 ISGylation和IFN-β产生的影响.
研究了NSP1α对TRIM25蛋白水平及其降解途径的影响.
确定NSP1α的蛋白酶活性是否对其对TRIM25的影响至关重要.

主要成果:

发现PRRSV NSP1α降低了TRIM25的ISGylation,并抑制了TRIM25介导的IFN-β的产生.
NSP1α通过降低TRIM25蛋白水平通过蛋白质酶体降解来促进PRRSV复制,而不会影响TRIM25转录.
由NSP1α降解TRIM25并不取决于其类似帕帕因的氨酸蛋白酶活性.

结论:

PRRSV NSP1α针对TRIM25进行降解,从而对抗宿主的抗病毒反应.
这项研究揭示了通过TRIM25降解来避免PRRSV免疫的新机制.
TRIM25被确定为PRRSV NSP1α的直接目标,突出了了解PRRSV病变的新途径.

关键词:

抗病毒反应抗病毒反应在 PRRSV NSP1α 中.蛋白质体系统是一种蛋白质体系统.在 TRIM2525 中,

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