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工程微血管网络使用KLF2报告器探测流量依赖的内皮细胞功能.

Adriana Blazeski1, Marie A Floryan2, Yuzhi Zhang3

  • 1Center for Excellence in Vascular Biology, Department of Pathology, Brigham and Women's Hospital, USA and Harvard Medical School, Boston, MA, USA; Cardiovascular Disease Initiative, Broad Institute of MIT and Harvard, Cambridge, MA, USA; Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.

Biomaterials
|July 6, 2024
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概括

带有KLF2流量传感器的工程微血管网络 (MVN) 揭示了血流如何影响血管结构和功能. 在3D系统中,流动促进了更大,更稳定的血管,改善了屏障功能和减少了血小板粘附.

关键词:
工程微血管网络的设计流媒体记者 流媒体记者流量传感器 流量传感器微流体芯片是一个微流体.剪切压力压力是什么

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科学领域:

  • 生物医学工程 生物医学工程
  • 血管生物学 血管生物学
  • 微流体学 微流体学

背景情况:

  • 血流造成的剪切应力调节了内皮细胞功能和血管结构.
  • 转录因子KLF2是内皮细胞对层状流的反应的关键,促进抗炎性表型.
  • 在流量诱导的变化中KLF2的作用在2D模型中得到了很好的研究,但在3D体外模型中没有.

研究的目的:

  • 开发和利用基于KLF2的流量传感器的工程微血管网络 (MVN).
  • 在3D中研究连续血流对血管结构和功能的影响.
  • 在3D血管系统中描述KLF2的流量依赖调节.

主要方法:

  • 工程微血管网络 (MVN) 包含基于KLF2的内皮细胞流量传感器在微流体芯片上.
  • 使用微流体的连续流量应用.
  • 对血管结构 (直径,分支),阻力,屏障功能和血小板粘附的分析.

主要成果:

  • 48小时的连续流量增加了KLF2在MVN中的记者表达.
  • 流量导致血管直径更大,血管分支减少,阻力降低.
  • 船舶直径对流量的反应独立于初始的MVN形态.
  • 暴露于流动的MVN表现出改善了血管屏障功能和降低了血小板粘附.

结论:

  • 带有KLF2传感器的工程MVN为研究3D血管系统的流动效应提供了一个新的工具.
  • 血液流动显著影响工程血管网络,促进稳定性和功能改善.
  • 这种3D模型提供了关于血管对剪切应激反应的见解,与生理学和疾病相关.