心脏蛋白质分析发现MYH6和COX5B是突然不明原因死亡的生物标志物
Ziyan Song1, Wensi Bian1, Junyi Lin1
1Department of Forensic Medicine, School of Basic Medical Sciences, Fudan University, Shanghai 200032, PR China.
Forensic science international
|July 6, 2024
概括
突然不明原因死亡 (SUD) 的诊断是具有挑战性的. 这项研究确定了心脏MYH6和COX5B蛋白质是SUD的有效双重生物标志物,提高了诊断准确度.
科学领域:
- 法医病理学 法医病理学
- 生物化学 生物化学
- 蛋白质组学是指蛋白质组学.
背景情况:
- 突然不明原因死亡 (SUD) 在法医病理学中存在诊断挑战,因为缺乏特定的生物标志物.
- 目前对SUD的诊断方法缺乏明确的指标,需要新的方法.
研究的目的:
- 在SUD病例中选差异表达蛋白 (DEPs).
- 验证已识别的DEP作为SUD诊断生物标志物的实用性.
- 使用蛋白质组数据开发SUD可靠的诊断策略.
主要方法:
- 在SUD,机械伤害和一氧化碳 (CO) 中毒病例中使用了无标签的蛋白质组分析,用于固定甲嵌入式 (FFPE) 心脏样本.
- 采用生物信息学和Adaboost-递归特征消除 (RFE) 来识别DEP中潜在的歧视性生物标志物.
- 在独立的SUD和对照队伍中,使用心脏组织分析和受体操作特征 (ROC) 分析验证了已识别的生物标志物 (MYH6,COX5B).
主要成果:
- 在SUD病例中确定了26个DEP; MYH6,COX5B和TNNT2被突出显示为潜在的歧视性生物标志物.
- 在独立的SUD病例中,MYH6和COX5B被证实是真正的DEP.
- MYH6和COX5B的组合实现了高诊断性能 (灵敏度为89.7%,特异性为84.4%,AUC为0.91).
- 使用这些生物标志物的诊断软件准确识别了87.5%的验证病例.
结论:
- 心脏MYH6和COX5B作为有效的双重诊断生物标志物,用于突然不明原因死亡.
- 开发的综合这些生物标志物的诊断策略为SUD诊断提供了一种实际的方法.
- 这项研究为识别SUD的法医病理学工具包提供了重大进步.
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