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作为一种基于口服蛋白质的候选疫苗,奇托桑-酸/R8三元聚电解质复合物诱导有效的粘膜免疫反应

Yiduo Liu¹, Meizhen Long¹, Yuanzhi Wang¹

¹National Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, No. 2 Yuanmingyuan West Road, Beijing 100193, PR China.

International journal of biological macromolecules

|July 6, 2024

概括

这项研究开发了一种新型的口服疫苗输送系统,使用基于多 (乳-co-glycolide) 酸的复合物. 该系统有效地输送抗原,增强免疫反应,并减少小鼠模型中的细菌负载.

科学领域:

生物技术是生物技术.
疫苗学疫苗学疫苗学
材料科学材料科学材料科学

背景情况:

口服疫苗接种为增强肠道和全身免疫提供了优势.
为预防传染病开发有效的口服亚单元疫苗配送平台至关重要.

研究的目的:

开发一种基于聚 (乳酸-co-glycolide) 酸 (PLGA) 的三元聚电解质复合物 (PEC) 用于口服抗原的输送.
评估这种PEC系统在保护抗原和增强免疫反应对抗Mycobacterium avium亚种结核病 (MAP) 的有效性.

主要方法:

使用PLGA,奇托,甲基酸盐和R8的三元PEC的配方.
在PEC中封装Mycobacterium avium亚种结核病 (MAP) 抗原 (HBf) 的封装.
在实验室中对模拟胃和肠液中的PEC稳定性的评估.
在MAP感染的小鼠模型中,对抗原吸收,免疫反应 (抗体,IgA,T细胞) 和治疗疗效的体内评估.

主要成果:

在模拟的胃液中,PEC表现出一种保护性的交联结构,并在模拟的肠液中分散为纳米粒子.
在小鼠中,口服三元PEC与R8增强了肠道淋巴状组织对抗原的吸收.
在受MAP感染的小鼠中,PEC促进了特定抗体和肠粘膜IgA分泌,增强了脏T细胞反应,减少了细菌负载和肝脏病理.

结论:

开发的三元PEC是口服亚单元疫苗的有希望的交付平台.
这种传递系统显示了预防和治疗MAP等感染的潜力,可能还有其他感染.

关键词:

基托桑是一种酸盐.口服疫苗候选人的口服疫苗甲基酸盐的使用方法通过膜的化物.

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