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Updated: Jun 21, 2025

Quantification of γH2AX Foci in Response to Ionising Radiation

Quantification of γH2AX Foci in Response to Ionising Radiation

Published on: April 6, 2010

作为对DNA损伤的反应,需要TORC2来积累γH2A

Adiel Cohen¹, Lea Lubenski², Ava Mouzon²

¹Department of Natural Sciences, The Open University of Israel, Ranana, Israel.

The Journal of biological chemistry

|July 6, 2024

概括

在酵母中,TORC2-Gad8信号传递对DNA损伤反应至关重要. 这一途径调节了对DNA修复至关重要的基因组H2A酸化,并涉及染色质修饰因子.

科学领域:

细胞生物学细胞生物学
分子生物学分子生物学
遗传学遗传学是一种遗传学.

背景情况:

托尔蛋白激酶是细胞生长,增殖和存活的中央调节者.
TORC2复合物及其下游激酶Gad8对于裂变酵母的应激反应至关重要.
基因组H2A酸化 (γH2A) 是DNA损伤的早期标志物.

研究的目的:

调查TORC2-Gad8信号传递在对DNA损伤和静止反应中的γH2A积累中的作用.
为了确定TORC2对γH2A的影响是直接的还是适应性的.

主要方法:

在Schizosaccharomyces pombe中使用了TOR特异性抑制剂Torin1.
在DNA损伤和静止下评估了γH2A积累.
研究了删除转录和染色质修饰因子的影响.

主要成果:

为了对DNA损伤和静止反应的γH2A积累,需要TORC2-Gad8信号传递.
在DNA受损时,TORC2对γH2A的影响是立即的.
缺少γH2A可以通过转录和染色质修饰因子的突变来挽救,包括Paf1C,SAGA,Mediator和域蛋白.

结论:

TORC2-Gad8信号传递通过调节γH2A.在DNA损伤反应途径中发挥着至关重要的作用.
TORC2-Gad8信号可能通过调节染色质结构和功能来影响γH2A积累.
这表明营养感应途径和DNA修复机制之间存在新的联系.

关键词:

对DNA损伤的反应反应这种植物是Schizosaccharomyces pombe.一个TOR复合体 (TORC)组织素H2A酸化基斯顿基因基因基因基因基因基因基因基因基因基因基因基因基因基因

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