相关实验视频

Updated: Jun 21, 2025

Differentiation of Monocytes into Phenotypically Distinct Macrophages After Treatment with Human Cord Blood Stem Cell CB-SC-Derived Exosomes

Differentiation of Monocytes into Phenotypically Distinct Macrophages After Treatment with Human Cord Blood Stem Cell CB-SC-Derived Exosomes

Published on: November 12, 2020

来自带血的外体细胞Treg细胞通过向单细胞促进糖尿病伤口愈合

Fan Yang¹, Donghua Cai¹, Ran Kong¹

¹National Clinical Research Center for Metabolic Diseases, Key Laboratory of Diabetes Immunology, Ministry of Education, Hunan Engineering Research Center of Cell Therapy for Diabetes, and Department of Metabolism and Endocrinology, The Second Xiangya Hospital of Central South University, Changsha, China.

Biochemical pharmacology

|July 6, 2024

概括

由带血调节性T细胞 (Treg) 衍生的外体体显示出加速糖尿病伤口愈合的前景. 这些外体促进单细胞两极分化,增强细胞迁移,提供潜在的无细胞治疗策略.

科学领域:

免疫学免疫学免疫学
再生医学是一种再生医学.
伤口治愈研究研究伤口治愈研究

背景情况:

慢性不愈合的糖尿病伤口是一个重大的临床挑战.
调节性T细胞 (Tregs) 调节炎症,影响伤口愈合.
外体,细胞释放的小囊泡,正在研究治疗潜力.

研究的目的:

调查带血 (CB) Tregs衍生的外体是否可以加速糖尿病伤口愈合.
探索CB-Treg外基因组通过哪些机制影响与伤口修复相关的细胞反应.

主要方法:

从CB-Treg培养物中分离出外体并进行了表征.
对外围血液单核细胞 (PBMC) 和单细胞进行了共同培养实验.
对纤维细胞和内皮细胞进行了体外迁移试验.
建立了一个使用外体荷载水凝治疗糖尿病伤口的小鼠模型.

主要成果:

CB-Treg外体,直径30-120纳米,表达了外体标记物 (TSG101,Alix,CD63) 的存在.
外体在体外促进了单细胞两极化到一种抗炎M2表型.
观察到内皮细胞和纤维细胞的增强迁移.
在体内,外体治疗加速了伤口愈合,减少了炎症,并增加了M2巨细胞.

结论:

来自CB-Treg的外体体代表了一种有前途的无细胞治疗方法,用于糖尿病伤口愈合.
治疗效果部分通过向单细胞和调节炎症反应来调节.
这一策略有可能改善慢性不愈合伤口患者的治疗结果.

关键词:

糖尿病伤口愈合的治疗方法异构体是什么? 异构体是什么? 外构体是什么?一个单细胞单细胞.监管T细胞监管T细胞

更多相关视频

Prospective, Randomized, and Controlled Study of a Human Umbilical Cord Mesenchymal Stem Cell Injection for Treating Diabetic Foot Ulcers

Prospective, Randomized, and Controlled Study of a Human Umbilical Cord Mesenchymal Stem Cell Injection for Treating Diabetic Foot Ulcers

Published on: March 3, 2023

A Protocol for Constructing a Rat Wound Model of Type 1 Diabetes

A Protocol for Constructing a Rat Wound Model of Type 1 Diabetes

Published on: February 17, 2023

相关概念视频

Clinical Applications of Epidermal Stem Cells

Clinical Applications of Epidermal Stem Cells

Epidermal stem cells (EpiSCs) are mainly located at the basal layer of the epidermis. These cells repair minor injuries of the skin and replace dead skin cells. However, EpiSCs’ cannot heal severe wounds such as major burns or those from diabetes or hereditary disorders. In such cases, culturing the epidermal stem cells from the patient is possible and has yielded successful treatment options, such as laboratory-grown skin grafts. These grafts are synthesized using a patient’s own...

Mesenchymal Stem Cells

Mesenchymal Stem Cells

Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...

iPS Cell Differentiation

iPS Cell Differentiation

The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.