从重要草药"Murraya koenigii"中分离出的Lactococcus lactis菌株的益生菌评估,粘附能力和安全性评估
Tholla Madana Shivani1, Mythili Sathiavelu2
1School of Biosciences and Technology, Vellore Institute of Technology, Vellore, 632014, India.
Scientific reports
|July 6, 2024
概括
从咖叶中分离出来的乳球菌 (MKL8) 显示出强大的益生菌潜力. 这种有益的细菌可以在恶劣的肠道条件下生存,粘附于细胞,抑制病原体,并且无毒,因此适合食品工业.
科学领域:
- 微生物学 微生物学
- 食品科学 食品科学 食品科学
- 益生菌 益生菌 益生菌
背景情况:
- 乳酸细菌 (LAB) 对肠道微生物群平衡至关重要.
- 穆拉亚koenigii (咖叶) 是潜在的益生菌菌株的来源.
- 评估LAB的益生菌潜力需要严格的体外测试.
研究的目的:
- 从Murraya koenigii中分离和描述LAB的益生菌特性.
- 评估一个孤立菌株的生存,坚持性,抗菌活性和安全性.
- 为了确定隔离剂适用于食品工业应用的适用性.
主要方法:
- 从Murraya koenigii.igii.中 LAB 的隔离.
- 在实验室中评估乳糖菌 (MKL8) 对,胆,低pH和透条件的耐受性.
- 评估MKL8对Caco-2细胞的粘附性,疏水性,自聚和抗菌活性.
- 使用MTT和血液溶解试验,确定MKL8的安全性.
主要成果:
- 在模拟胃状况 (>72%) 和高胆汁度 (>79%) 中,MKL8显示出显著的生存率.
- 隔离物表现出强烈的疏水性 (85%),自聚 (87.3%-91.7%) 和粘附于Caco-2细胞.
- 在MTT和血溶性试验中,MKL8显示出无毒性特征,并抑制了测试的病原体.
结论:
- 乳球菌 (MKL8) 具有强大的益生菌特性,包括胃肠道耐受性和病原体抑制.
- 菌株的安全性和粘附能力支持其作为功能成分的潜力.
- MKL8是食品行业应用的有希望的候选人,以提高肠道健康.
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