Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

MAFF regulates ferroptotic sensitivity through iron homeostasis and fatty acid synthesis.

Cell death & disease·2026
Same author

Multiscale Radiobiological Assessment of Laser-Driven Very High Energy Electrons Versus Conventional Electrons.

Advances in radiation oncology·2026
Same author

autoFISH: a modular toolbox for sequential single-molecule RNA FISH experiments.

Communications biology·2026
Same author

Sparing effects of FLASH irradiation in patient-derived lung tissue.

Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology·2025
Same author

Conserved signals control self-organization and symmetry breaking of murine bilayered epithelia during development and regeneration.

Developmental cell·2025
Same author

Homebuilt Imaging-Based Spatial Transcriptomics: Tertiary Lymphoid Structures as a Case Example.

Methods in molecular biology (Clifton, N.J.)·2024
Same journal

Ciliary flow and morphology shape mass transport at the surface and within gastrovascular cavities of black corals.

Communications biology·2026
Same journal

Virus-mediated prokaryotic community adaptation dynamics under thermal stress in municipal organic solid waste microbiomes.

Communications biology·2026
Same journal

Multi-omics insights into the woolly trait of Saussurea medusa and the plant's coordinated regulation of flavonoid biosynthesis.

Communications biology·2026
Same journal

Loss contexts enhance dorsolateral prefrontal interpersonal neural synchrony during successful human deceptive recommendations.

Communications biology·2026
Same journal

Neuro-regulator role of H<sub>2</sub>S in astrocyte activation and its effects on neurological damage and behavior of VPA-exposed rats.

Communications biology·2026
Same journal

Temporal orchestration of transcriptional and epigenomic programming underlying maternal embryonic diapause in a cricket model.

Communications biology·2026
查看所有相关文章

相关实验视频

Updated: Jun 9, 2026

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues
10:12

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Published on: January 10, 2019

18.5K

一个点云细分框架用于基于图像的空间转录学.

Thomas Defard1,2,3,4,5, Hugo Laporte6,7, Mallick Ayan6

  • 1Centre for Computational Biology (CBIO), Mines Paris, PSL University, 75006, Paris, France.

Communications biology
|July 6, 2024
PubMed
概括
此摘要是机器生成的。

康塞格 (ComSeg) 是一种用于在空间RNA分析数据中对细胞进行细分的新算法. 它准确地识别细胞而不需要膜标记物,改善细胞类型,调用复杂的组织.

更多相关视频

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
09:19

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

4.9K
Spatially Compact Arrangement of Larval Zebrafish Sections for Spatial Transcriptomic Analysis
07:40

Spatially Compact Arrangement of Larval Zebrafish Sections for Spatial Transcriptomic Analysis

Published on: May 16, 2025

157

相关实验视频

Last Updated: Jun 9, 2026

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues
10:12

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Published on: January 10, 2019

18.5K
Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
09:19

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

4.9K
Spatially Compact Arrangement of Larval Zebrafish Sections for Spatial Transcriptomic Analysis
07:40

Spatially Compact Arrangement of Larval Zebrafish Sections for Spatial Transcriptomic Analysis

Published on: May 16, 2025

157

科学领域:

  • 空间转录组学 空间转录组学
  • 计算生物学是一种计算生物学.
  • 生物信息学是一种生物信息学.

背景情况:

  • 基于图像的空间RNA分析为组织组织提供了高分辨率的洞察力.
  • 将RNA转录分配给单个细胞对于下游分析至关重要,例如细胞类型识别.
  • 精确的细胞细分在组织数据中具有挑战性,特别是没有可靠的膜标记物.

研究的目的:

  • 介绍ComSeg,一个用于空间RNA分析数据的新型细分算法.
  • 开发一种直接对RNA位置进行操作的方法,独立于细胞形状先验.
  • 为了实现精确的细胞细分在复杂的组织与多样化的细胞形态.

主要方法:

  • 康塞格算法直接运行在单个RNA位置上.
  • 不依赖于隐式或显式的细胞形状先验.
  • 适用于具有任意细胞形状的复杂组织.

主要成果:

  • 与现有的最先进的方法相比,ComSeg表现出优越的性能.
  • 在现场单细胞RNA分析和细胞类型调用方面,其表现优于当前的方法.
  • 在模拟和实验数据集上验证.

结论:

  • 在空间RNA分析中,ComSeg为细胞细分提供了有效的解决方案.
  • 允许更准确的现场细胞类型调用复杂的生物样本.
  • 该算法作为一个开源软件包可供更广泛的研究使用.