在真实样本中使用光谱光度云点提取法量化的量化,用于与阿斯特拉松色G形成的离子对的光谱光度云点提取
Abdullah A A Sari1, Salah M El-Bahy2, Khaled F Debbabi3
1Department of Chemistry, University College in Al-Jamoum, Umm Al-Qura University, 21955 Makkah, Saudi Arabia.
概括
一种新的云点提取方法可以准确地确定食品和饮料中的超微量物种. 这种光谱光度计技术提供了一种灵敏而可靠的方法,用于在现实世界样本中检测致癌.
科学领域:
- 分析化学 分析化学
- 环境化学环境化学
背景情况:
- 由于致癌性,消耗品中的物种分析至关重要.
- 超微量的确定需要敏感和选择性的方法.
研究的目的:
- 开发一种新的云点提取 (CPE) 方法,用于超微量物种的确定.
- 在水,饮料和食品等实物样本中分析的光谱光度检测程序.
主要方法:
- 云点提取 (CPE) 使用选择性三元复合体As(V与阿斯特拉松色G (AOG+), tartaric 酸和Triton X-114在pH值4.0时.
- 对的光谱测定.
- 方法验证通过与石墨炉原子吸收光谱法 (GF-AAS) 的比较.
主要成果:
- 获得了200的预度因子和0.88 ng/mL的检测极限,用于As(V).
- 在3.0-160 ng/mL范围内观察到的校准曲线线性,R2 = 0.9988.8.
- 高分子吸收率 (4.38 × 10^5 L/mol cm) 和桑德尔灵敏度 (0.018 ng cm−2) 的高.
- 统计分析 (t-测试和F-测试) 与GF-AAS相比没有显著差异.
结论:
- 开发的CPE光谱测量方法是有效的超微量在真实样本的确定.
- 该方法表现出高灵敏度,选择性和准确性,与GF-AAS.等既定技术相提并论.
- 这种新的方法为监测食品和饮料中污染提供了可靠的工具.
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