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循环中的与肺癌相关的非编码RNA与职业接触六价有关 - - 在SafeChrom项目内的横截面研究

Zheshun Jiang¹, Romane Person², Thomas Lundh¹

¹Division of Occupational and Environmental Medicine, Department of Laboratory Medicine, Lund University, Lund, Sweden.

Environment international

|July 7, 2024

概括

六价 (Cr(VI)) 暴露与肺癌工作者的循环非编码RNA (ncRNA) 的较低水平有关. 这些ncRNAs可以作为Cr(VI) 毒性的生物标志物及其在致癌中的作用.

科学领域:

环境健康环境健康
分子生物学分子生物学
癌症研究癌症研究

背景情况:

六价 (Cr(VI)) 是已知的人类致癌物,增加肺癌风险.
非编码RNAs (ncRNAs) 在肺癌发展中的作用已经确立,但它们与Cr(VI) 暴露的联系不太清楚.

研究的目的:

量化与肺癌相关的循环ncRNA表达在Cr (VI) 暴露的工人与对照.
为了研究ncRNA水平和红细胞 (RBC) 和尿液中的度之间的关系.
探索不同ncRNA之间的相关性.

主要方法:

使用实时定量PCR测量长非编码RNAs (lncRNAs) 和microRNAs (miRNAs) 在111名暴露于CrVI的工人和72名对照者的血中.
在RBC (长期暴露) 和尿液 (短期暴露) 中评估了水平.
具体的测定针对三个lncRNAs (H19,MALAT1,NORAD) 和四个miRNAs (miR-142-3p,miR-15b-5p,miR-3940-5p,miR-451a).

主要成果:

与对照组相比,暴露于Cr (VI) 的工人中MALAT1,NORAD和所有四种小RNA的表达显著较低.
减少的ncRNA表达与较高的RBC-Cr度相关,表明了剂量-反应关系.
多变量分析证实暴露个体的lncRNA和miRNA水平较低,即使经过对混因素的调整.

结论:

的暴露与循环的ncRNA表达相反地相关,以剂量依赖的方式.
ncRNAs显示出作为CrVI诱导毒性的生物标志物的潜力.
lncRNAs和miRNAs之间的相互作用表明它们参与了对Cr(VI) 致癌的关键调节途径.

关键词:

生物标志物生物标志物六价的六价是一种肺癌是一种肺癌.在RBC中使用.在ncRNA中,我们可以看到这是一个小RNARNA.

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