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基于系统生物学和细胞实验,探索黄素改善系统性硬化症中免疫和炎症反应的机制

Xiang Ding¹, Jian Liu², Xiaolu Chen¹

¹First Clinical Medical College, Anhui University of Traditional Chinese Medicine, Hefei, Anhui 230031, PR China; Department of Rheumatology and Immunology, First Affiliated Hospital of Anhui University of Traditional Chinese Medicine, Shushan, Hefei, Anhui 230038, PR China.

International immunopharmacology

|July 7, 2024

概括

传统中医 (TCM) 通过抑制TNF/NF-κB通路来缓解系统性硬化症 (SSc) 炎症. 作为TCM的关键成分,素通过向SSc-人类皮肤纤维细胞中的这种途径来表现出抗炎作用.

科学领域:

免疫学免疫学免疫学
药理学药理学是指药理学的学科.
综合医学是一个整体的医学.

背景情况:

系统性硬化症 (SSc) 是一种以炎症为特征的免疫媒介疾病.
传统中医 (TCM) 越来越多地被用于治疗免疫疾病.
了解SSc中TCM的抗炎机制至关重要.

研究的目的:

为了阐明在SSc.中TCM成分黄素的抗炎机制.
为了确定SSc炎症的核心TCM处方和活性成分.
调查TNF/NF-κB通路在黄素治疗效果中的作用.

主要方法:

用文献计量分析,ELISA,数据挖掘和网络药理学来确定TCM的处方和目标.
分子对接评估了luteolin与TNF和NF-κB的结合亲和力.
在体外研究 (ELISA,RT-PCR,西部斑,免疫光) 评估了黄素对SSc-人类皮肤纤维细胞 (HDF) 和TNF/NF-κB通路的影响.

主要成果:

确定了核心的TCM处方和黄素作为一种关键的抗炎成分.
卢铁表明与TNF和NF-κB有很强的结合.
卢铁抑制了SSc-HDF中TNF/NF-κB通路的激活,减少了炎症性细胞因子.
PKM2增强了黄素的抗炎作用.

结论:

通过抑制TNF/NF-κB通路,TCM有效地减轻SSc炎症.
氨酸是TCM中治疗SSc炎症的关键活性成分.
这项研究为TCM在SSc中的治疗应用提供了机制基础.

关键词:

人类表皮纤维细胞.免疫性炎症是一种免疫性炎症.卢特奥林是什么? 卢特奥林是什么?系统性硬化症系统性硬化症这是TNF/NF-κBB.传统的中国医学是传统的中国医学.

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