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氨基基基改性聚乙烯纳米塑料诱导了Artemia血细胞的多重反应

Hu Duan¹, Namin Pan², Xuanxuan Shao²

¹Asian Regional Artemia Reference Center, Tianjin Key Laboratory of Marine Resources and Chemistry, College of Marine and Environmental Sciences, Tianjin University of Science and Technology, Tianjin 300457, China; Key Laboratory of Marine Resource Chemistry and Food Technology (TUST), Ministry of Education Tianjin University of Science and Technology, Tianjin 300457, China.

Comparative biochemistry and physiology. Toxicology & pharmacology : CBP

|July 7, 2024

概括

聚乙烯纳米塑料 (PS-NH2 NPs) 伤害水生无脊椎动物,如Artemia. 暴露会影响他们的免疫细胞,基因表达和肠道微生物,这表明Artemia血细胞可以表明纳米塑料污染水平.

科学领域:

环境毒理学环境毒理学
水生无脊椎动物免疫学
纳米毒理学研究

背景情况:

聚乙烯纳米塑料 (PS-NH2NP) 对水生生态系统构成重大风险.
水生无脊椎动物对纳米塑料的先天免疫反应尚未得到充分理解.
关键的动物浮游生物之一Artemia是研究水生有毒性的模型生物.

研究的目的:

研究PS-NH2NP对盐水Artemia的血液学和免疫学影响.
阐明暴露在纳米塑料中的Artemia天生的免疫机制.
评估Artemia血细胞作为纳米塑料污染的生物标志物的潜力.

主要方法:

在体内和体外暴露Artemia的氨基基改性聚烯纳米塑料 (PS-NH2NP).
确定致命度 (LC50) 和评估血细胞亚群和细胞.
传输电子显微镜 (TEM) 用于超结构分析和基因表达造型 (RT-qPCR).

主要成果:

PS-NH2 NPs对菌有显著的毒性,LC50值为1.09 μg·mL−1 (48h) 和0.42 μg·mL−1 (7d).
TEM揭示了细胞损伤,包括细胞质真空化和线粒体胀.
暴露改变了血液图,增加了细胞活动,调节了免疫和亡相关的基因表达,并破坏了血淋巴微生物群.

结论:

在Artemia血细胞中,PS-NH2NP诱导多方面的压力反应,影响细胞完整性,免疫基因表达和微生物平衡.
亚特米亚血细胞显示出作为评估水生环境中纳米塑料污染的敏感生物指标的潜力.
这些发现有助于了解水生无脊椎动物中纳米塑料诱导的血液毒性机制.

关键词:

动脉动脉症是什么动脉动脉症血液图的变化变化纳米塑料是一种纳米塑料.压力反应应激反应有毒性有毒性

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