宿主基因表达的抑制与潜在的结核病感染有关:一种可能的诊断生物标志物
Ritah Nakiboneka1,2,3,4,5, Nicolò Margaritella6, Tonney Nyirenda2
1Division of Infection and Global Health, School of Medicine, University of St Andrews, St Andrews, UK.
Scientific reports
|July 7, 2024
概括
新的转录基因标记物 (TM) 显示出在家庭接触者中诊断潜伏结核病感染 (LTBI) 的前景. 抑制的基因表达可能是LTBI比目前的标准测试更敏感的生物标志物.
科学领域:
- * 传染病 传染病
- * 分子诊断学 分子诊断学
- * 结核病研究研究
背景情况:
- *世界卫生组织的结核病终结战略的目标是到2035年将结核病 (TB) 发病率降低90%.
- *对活跃结核病患者的接触者进行潜在结核病感染 (LTBI) 的系统检测和治疗对于减少结核病发病率至关重要.
- *目前对LTBI的准确诊断工具有限.
研究的目的:
- * 评估全血宿主转录基因标记 (TM) 以诊断活跃结核病患者的家庭接触者中的LTBI.
- * 为了比较TM的诊断准确性与QuantiFERON-TB Gold Plus干扰素玛释放试验 (IGRA).
主要方法:
- * 细菌学证实结核病例的家庭接触者 (TBEx) 和艾滋病毒阴性健康对照 (HC) 被纳入.
- *使用QuantiFERON-TB Gold Plus IGRA来确定LTBI.
- * 全血宿主转录基因标记 (TM) 表达量化使用逆转录酶定量PCR.
主要成果:
- *11个评估的TM在LTBI患者中显示出明显抑制的表达,与HC患者相比.
- * ZNF296和KLF2的表达与LTBI密切相关,并将LTBI与HC区分开来.
- *显著比例的IGRA阴性结核病暴露个体表现出类似于IGRA阳性个体的抑制TM表达模式.
结论:
- * 全血中抑制的基因表达可能代表着比IGRA更敏感的LTBI生物标志物.
- *像ZNF296和KLF2这样的转录组标志物有可能改善LTBI诊断.
- *需要进一步的研究来验证这些TM作为LTBI的诊断生物标志物.
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