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用复合性Saccharomyces cerevisiae进行浸泡免疫,显示ORF25诱导的对cyprinid疹病毒2的保护性免疫力

Maoxia Yang^1,2,3, Sheng Luo^1,2,3, Qianjin Zhou^1,2,3

¹State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, School of Marine Sciences, Ningbo University, Ningbo, China.

Journal of fish diseases

|July 8, 2024

概括

这项研究开发了一种新的浸泡疫苗,使用显示Cyprinid疹病毒2 (CyHV-2) 抗原的酵母. 该疫苗有效地保护鱼免受CyHV-2感染,证明了其在预防鱼类疾病方面的潜力.

科学领域:

水产养殖是水产养殖的一种方式.
鱼类免疫学鱼类免疫学
疫苗的研发工作正在进行中.

背景情况:

使用酵母显示的口服疫苗很常见,但浸泡疫苗的探索较少.
类疹病毒2 (CyHV-2) 对鱼群构成重大威胁.

研究的目的:

为了评估基于Saccharomyces cerevisiae显示CyHV-2 ORF25.25的浸泡疫苗的疗效.
评估Carassius auratus gibelio中对CyHV-2的免疫反应和保护作用.

主要方法:

通过在Saccharomyces cerevisiae上显示ORF25来准备一个浸泡疫苗.
通过浸泡接种Carassius auratus gibelio疫苗,然后接种一次强剂量.
测量了ORF25特异性抗体水平和免疫基因表达 (IgM,IgT,IL-1β,IFN-1).
评估了对CyHV-2挑战的保护,包括生存率,病毒复制和组织学损伤.

主要成果:

在免疫鱼血清中检测到高水平的ORF25特异性抗体.
在头和脏中对先天性和适应性免疫基因 (IgM,IgT,IL-1β,IFN-1) 的升级调节.
在受到CyHV-2挑战的鱼类中实现了50.2%的相对百分比生存率.
在接种疫苗的鱼中,病毒复制和组织学损伤明显减少.

结论:

在Saccharomyces cerevisiae中显示的ORF25是针对CyHV-2的浸泡疫苗的有希望的候选者.
浸泡疫苗有效诱导鱼类的先天性和适应性免疫力.
这种方法为防止水产养殖中CyHV-2感染提供了一个可行的策略.

关键词:

这种病毒名为CyHV-2.在ORF25的ORF25 克鲁西安鱼是一种鱼.沉浸式疫苗的疫苗.酵母表面显示器显示酵母表面.

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