Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and the...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Enhancing Vibrational Spectroscopy-Based Diagnosis through Bottom-Up Modeling: The Case of Infrared Absorption Spectrum of Urine.

Analytical chemistry·2026
Same author

Proteasome Inhibition Amplifies Endoplasmic Reticulum (ER) Stress Responses: Comparative Proteomics of Chinese Hamster Ovary Cell Lines.

Biomolecules·2026
Same author

Raman spectroscopic monitoring of multiproduct chemical reaction kinetics; the case of ester hydrolysis.

The Analyst·2026
Same author

Raman spectroscopic profiling of cell death induced by cytotoxic copper(II), manganese(II), and silver(I) complexes containing dicarboxylate and 1,10-phenanthroline ligands: novel insights into in vitro mechanisms.

Metallomics : integrated biometal science·2026
Same author

Elucidating time-resolved intracellular metabolic dynamics <i>via</i> label-free Raman microspectroscopy and 2D correlation spectroscopy.

The Analyst·2025
Same author

Correction: Thenuwara et al. Recent Advancements in Lateral Flow Assays for Food Mycotoxin Detection: A Review of Nanoparticle-Based Methods and Innovations. <i>Toxins</i> 2025, <i>17</i>, 348.

Toxins·2025

相关实验视频

Updated: Jul 17, 2026

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy
13:48

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy

Published on: May 29, 2012

17.0K

使用拉曼显微光谱学对二维和三维基板上的干细胞分化的比较分析.

F Ravera1, E Efeoglu2, H J Byrne1

  • 1FOCAS Research Institute, Technological University Dublin, City Campus, Dublin 8, Ireland. D16126527@mytudublin.ie.

The Analyst
|July 8, 2024
PubMed
概括

与二维培养相比,三维 (3D) 原水凝显著增强介质干细胞 (MSC) 原生. 拉曼显微镜 (RMS) 在3D环境中有效监测这种软骨形成过程.

科学领域:

  • 生物医学工程 生物医学工程
  • 细胞生物学 细胞生物学
  • 生物材料科学 生物材料科学

背景情况:

  • 混凝土生成,即介质干细胞 (MSCs) 分化为混凝土细胞的过程,对于软骨形成至关重要.
  • 三维 (3D) 培养系统为研究细胞发育提供了比传统的二维培养更具生理相关的环境.
  • 在3D中了解体生成对于开发有效的软骨修复疗法至关重要.

研究的目的:

  • 为了评估3D原体I型水凝作为大鼠MSCchondrogenesis的基质.
  • 为了比较3D水凝与2D单层培养中的体差异化速率和分子特征.
  • 为了证明拉曼显微光谱 (RMS) 在3D培养系统中监测体生成的实用性.

主要方法:

  • 从老鼠骨髓衍生出的MSCs在3D原蛋白I型水凝和2D单层中培养了3周.
  • 使用无标签拉曼显微镜 (RMS) 监测体差异化,以分析亚细胞光谱特征.
  • 高密度微量培养物也被研究以了解细胞矩阵相互作用.

主要成果:

  • 3D水凝中的MSC与2D培养中的MSC相比,表现出明显更高的冠状体差异化率.
  • 3D培养显示出更强烈和更均的表达chondrogenesis标记物,如原蛋白,糖氨基甘氨酸 (GAGs) 和农草.

更多相关视频

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes
10:48

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes

Published on: April 12, 2015

10.0K
An Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects
07:37

An Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects

Published on: January 9, 2020

9.4K

相关实验视频

Last Updated: Jul 17, 2026

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy
13:48

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy

Published on: May 29, 2012

17.0K
Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes
10:48

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes

Published on: April 12, 2015

10.0K
An Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects
07:37

An Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects

Published on: January 9, 2020

9.4K
  • RMS检测到蛋白质和脂质的明显光谱变化,表明在3D环境中成功分化.

    • 结论:

    • 3D I型原蛋白水凝是体外软体生成研究的有希望的基质.
    • 拉曼显微镜是一种有价值的,非破坏性的工具,用于监测复杂的3D培养环境中的体生成.
    • 3D培养系统加速和增强MSC的原体分化,为软骨再生疗法提供了潜力.