相关实验视频

Updated: Jun 21, 2025

RNA Interference in Aquatic Beetles as a Powerful Tool for Manipulating Gene Expression at Specific Developmental Time Points

RNA Interference in Aquatic Beetles as a Powerful Tool for Manipulating Gene Expression at Specific Developmental Time Points

Published on: May 29, 2020

一个染色体水平的参考基因组组合和巨型淡水 (Macrobrachium rosenbergii) 的全长转录组合

Wirulda Pootakham¹, Kanchana Sittikankaew¹, Chutima Sonthirod¹

¹National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Pathum Thani 12120, Thailand.

G3 (Bethesda, Md.)

|July 8, 2024

概括

研究人员已经对巨型淡水的基因组进行了测序,提供了染色体规模的参考和全长的转录组. 该资源将有助于了解的生物学,并改善水产养殖养殖计划以获得有价值的特征.

科学领域:

水产养殖是水产养殖的一种方式.
基因组学就是基因组学.
文字转录学 (Transcriptomics) 是一个学科.

背景情况:

巨型淡水 (Macrobrachium rosenbergii) 对全球水产养殖至关重要.
在M. rosenbergii形态类型中的遗传复杂性带来了种植挑战.

研究的目的:

为了产生第一个染色体尺度参考基因组M. rosenbergii.
为了创建一个高质量的全长的转录组组合M. rosenbergii.

主要方法:

用于草稿组装的PacBio高真实性 (HiFi) 测序.
染色体接触映射 (Hi-C) 用于在染色体尺度上的脚手架.
使用基因组和转录组数据分析替代拼接事件.

主要成果:

一个3.73Gb的染色体规模的基因组组件,N50支架长度为33.6Mb.
重复的元素占基因组的近60%.
确定了2041个替代拼接事件,其中表细胞跳转是最常见的.

结论:

染色体水平的基因组和转录组组件对于M. rosenbergii生物研究至关重要.
这些资源将促进分子育种计划,以获得水产养殖中理想的特征.
促进商业水产养殖改进的巨型淡水品种的开发.

关键词:

马克罗布拉基乌姆罗森伯基基这就是Hi-C.在ISO-seqq中使用.替代性拼接是一种替代性的拼接.染色体规模的基因组组合组件转录组 (transcriptome) 是一个转录组.

更多相关视频

The Barnacle Balanus improvisus as a Marine Model - Culturing and Gene Expression

The Barnacle Balanus improvisus as a Marine Model - Culturing and Gene Expression

Published on: August 8, 2018

A Converging Strategy for the Generation of a Virtually Sequenced cDNA Library from Unreferenced Pacific Oysters

A Converging Strategy for the Generation of a Virtually Sequenced cDNA Library from Unreferenced Pacific Oysters

Published on: June 13, 2019

相关概念视频

Genome Annotation and Assembly

Genome Annotation and Assembly

The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.

Ribosome Profiling

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...

RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...