一级衍生光谱法用于同时确定维生素C和尼古丁胺胺:在晶的定量分析中的应用
Clóvis A Balbinot Filho1, Renata F Teixeira1, Jônatas L Dias1
1Department of Chemical and Food Engineering, Federal University of Santa Catarina, UFSC, PO Box 476, 88040-900 Florianópolis, SC, Brazil.
ACS omega
|July 8, 2024
概括
一种新的光谱测定方法可以准确量化维生素C (l-亚斯科布酸) 和尼古丁胺在晶中的含量. 这种方法为评估共晶纯度提供了具有成本效益和效率的替代方案.
科学领域:
- 分析化学 分析化学
- 材料科学 材料科学 材料科学
- 制药科学 制药科学
背景情况:
- 维生素C (l-ascorbic acid,ASC) 和尼古丁胺 (NIC) 通过键形成共晶体.
- 准确量化共晶成分和纯度是具有挑战性的,因为重叠的紫外线吸收和ASC不稳定性.
- 现有的光谱光度方法面临着狭窄的波长范围和维生素降解的局限性.
研究的目的:
- 开发一种快速,简单和可靠的光谱测定方法,用于同时量化共晶体中的ASC和NIC.
- 建立一种评估ASC-NIC共晶的纯度的方法.
- 为现有分析技术提供一个具有成本效益和效率的替代方案.
主要方法:
- 利用一级衍生光谱仪 (FODS) 进行ASC和NIC的同时确定.
- 在分析过程中使用酸盐作为ASC的稳定剂.
- 使用标准添加技术验证了FODS方法,并将结果与LC-MS和固态方法 (PXRD,DSC) 进行了比较.
主要成果:
- 该FODS方法证明了线性 (2-24μg·mL-1) 和良好的精度.
- 高回收率 (ASC为96.5-102.4%,NIC为95.3-101.9%) 证实了方法的准确性.
- 根据FODS (58-100%) 确定的晶纯度与LC-MS结果 (62-100%) 非常相匹配,准确度超过97%.
结论:
- FODS方法提供了一种灵敏,准确和可靠的方法,用于同时量化共晶体中的ASC和NIC.
- 这种方法是HPLC和经典固态方法的经济有效,高效和合适的替代方法,用于评估共晶的纯度.
- 开发的试验解决了维生素结晶分析中快速和精确量化的需求.
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