脉动可以通过激活FXR-ASBT通路来缓解DSS诱导的UC,以改善失调的胆酸平衡
Ying Xiao1, Ya-Qian Jia1, Wen-Juan Liu1
1College of Veterinary Medicine, Institute of Traditional Chinese Veterinary Medicine, Gansu Agricultural University, Lanzhou, China.
Frontiers in pharmacology
|July 8, 2024
概括
脉动 (PD) 通过恢复胆酸平衡和减少炎症,有效治疗性结肠炎 (UC). 这项研究突出了PDPD.
科学领域:
- 药理学和胃肠病学 药理学和胃肠病学
- 传统中国医药 传统中国医药
- 分子生物学分子生物学
背景情况:
- 性结肠炎 (UC) 是一种慢性炎症性疾病.
- 脉冲 (Pulsatilla decoction,PD) 是一种传统的治疗UC的方法.
- FXR和ASBT通路对于胆酸平衡和UC进展至关重要.
研究的目的:
- 调查PD对DSS诱导的UC的保护机制.
- 为了阐明PD对Farnesoid X受体 (FXR) 和依赖胆酸转运体 (ASBT) 途径的调制.
主要方法:
- 在小鼠中用硫酸盐 (DSS) 诱导的急性UC模型.
- 评估疾病活动指数 (DAI),结肠组织病理学,杯细胞计数和炎症性细胞因子.
- 通过PCR阵列和Western blotting分析FXR-ASBT通路中的基因和蛋白质表达.
主要成果:
- 治疗PD显著降低了DAI得分和结肠损伤.
- PD调节了炎症,杯状细胞数量,并恢复了胆酸平衡.
- 治疗PD恢复了FXR,GPBAR1,CYP7A1,FGF15的蛋白质表达,并显示了ABCB11和RXRα的趋势.
结论:
- 在UC的小鼠模型中,PD显示出显著的治疗效果.
- PD的有效性与调节胆酸平衡和肠道屏障功能有关.
- PD减轻了肠道炎症,为UC提供了潜在的治疗策略.
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