从Kushui中提取和分离的安东素是通过乙醇-NH (NH) SO (SO) 水性两相系统
Yuanyuan Li1, Tongyu Li1, Hongxu An1
1Jingjiang College, Jiangsu University Zhenjiang 212013 PR China liyuanyuan@ujs.edu.cn.
RSC advances
|July 8, 2024
概括
这项研究优化了使用乙醇-硫酸盐水性双相系统 (ATPS) 来从库舒伊中提取安托西亚宁,实现了高回收和糖去除. 开发的ATPS方法有效地分离了有价值的安托氨酸.
科学领域:
- 食品科学 食品科学 食品科学
- 生物化学 生物化学
- 分离科学 分离科学
背景情况:
- 安西是有价值的天然颜料,具有显著的健康益处.
- 从昆等植物来源有效提取和净化反素对于它们的应用至关重要.
- 传统的提取方法可能会面临挑战,即同时将素与糖等其他化合物分开.
研究的目的:
- 开发和优化一种水性双相系统 (ATPS),用于同时提取安素和从Kushui中去除糖.
- 调查各种参数对ATPS效率的影响.
- 确定最大限度地回收和纯度的最佳条件.
主要方法:
- 使用乙醇-硫酸盐水性双相系统 (ATPS) 进行同时提取和糖去除.
- 研究了盐类型,盐和乙醇度,温度和pH的影响.
- 采用Box-Behnken设计响应表面方法 (RSM) 来优化提取参数.
主要成果:
- 酸提取的最佳条件被确定为22% (w/w) 硫酸,25% (w/w) 乙醇,pH 5和33.5°C.
- 在顶级阶段,达到了最大的分割系数5.64和78%的反素回收率.
- 证明了有效的糖去除,在底部阶段的最大回收率为70.09%.
- 通过HPLC分析证实了通过ATPS提取的安托西亚宁与传统提取的相似性,验证了ATPS的适用性.
结论:
- 乙醇-硫酸盐ATPS是一种适合和有效的方法,用于同时提取安东和从库舒伊中去除糖.
- 使用RSM确定了最佳的提取条件,从而导致高氨酸回收.
- 在低pH值和低温度下储存时,从库舒伊中提取的安托是最稳定的.
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