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Updated: Jun 21, 2025

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在表达CRISPR/Cas9的野外和温室种植树木中发现了罕见但多样化的非标和体质突变

Greg S Goralogia¹, Isabella M Andreatta¹, Victoria Conrad¹

¹Department of Forest Ecosystems and Society, Oregon State University, Corvallis, OR, United States.

Frontiers in bioengineering and biotechnology

|July 8, 2024

概括

在树和白树中进行CRISPR基因编辑显示了非常低的非目标突变率,与自然突变率相当. 这表明CRISPR/Cas9对长寿植物如树木是安全的.

科学领域:

植物生物技术植物生物技术
基因组学就是基因组学.
基因编辑基因编辑

背景情况:

克里斯普尔基因编辑提供了精确的突变,但存在异常突变的风险.
在克隆繁殖的植物中,由于编辑试剂的长时间存在,这种风险被放大.

研究的目的:

评估CRISPR/Cas9目标外突变的频率和特征在各种树种群中 (Populus和Eucalyptus).
评估CRISPR/Cas9技术在长寿木质植物中的安全性和可靠性.

主要方法:

针对性测序~20,000个基因组位点,最多5个基因对同质,以指导RNA (gRNA) 目标位点.
从37个独立的CRISPR/Cas9转基因插入事件中分析了96个树样本,使用6个独特的gRNA.

主要成果:

观察到非常低的目标外突变率:树的1.2 × 10−9和树的3.1 × 10−10.
目标以外的突变是特异性的,不仅仅是由序列相似性预测的,有些网站有多达五个不匹配.
与PAM站点相对的突变位置反映了目标编辑模式.

结论:

树木中的CRISPR/Cas9脱基突变率可以忽略不计,支持其他植物物种的发现.
这项研究验证了CRISPR/Cas9作为长寿植物基因改造的安全有效工具,即使转基因存在多年.

关键词:

克里斯普尔是什么意思?克里斯普尔是什么意思?树是什么意思? 树是什么意思人口的人民 (Populus Populus) 是一个基因编辑基因编辑在目标之外编辑.树木生物技术树木生物技术

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