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软化突变的Salmonella enterica Typhimurium通过免疫反应介导黑色素瘤回归

Genesy Pérez Jorge^1,2, Marco Gontijo^1,3, Marina Flóro E Silva^1,4

¹Departamento de Genética, Evolução, Microbiologia e Immunologia, Instituto de Biologia, Universidade Estadual de Campinas-UNICAMP, Campinas, SP, Brazil.

Experimental biology and medicine (Maywood, N.J.)

|July 8, 2024

概括

沙门氏菌EntericTyphimurium突变体显示希望作为癌症免疫疗法. 减弱的DihfABpmi菌株通过激活抗瘤免疫反应,有效地减少了黑色素瘤瘤.

科学领域:

在瘤学瘤学.
微生物学微生物学
免疫学免疫学免疫学

背景情况:

癌症发病率上升需要新的治疗策略.
沙门氏菌 (Salmonella enterica Typhimurium) 免疫疗法是一个有前途的抗癌方法.
减弱的细菌菌株对于安全有效的癌症治疗至关重要.

研究的目的:

评估两种S. enterica Typhimurium突变体 (ΔtolRA和ΔihfABpmi) 的衰减和抗瘤疗效.
在小鼠黑色素瘤模型中研究这些突变的治疗潜力.

主要方法:

使用Galleria mellonella模型对细菌衰减的评估.
在瘤细胞中评估细菌入侵和生存.
在小鼠黑色素瘤模型中进行体外和体外抗瘤活性测试.
免疫细胞概况 (巨分化) 和炎症媒介和亡标记物的基因表达的分析.

主要成果:

德托拉突变体表现出高衰减,但抗瘤效果较弱.
减弱的DihfABpmi菌株在6天内在小鼠中显示出显著的瘤回归.
ΔihfABpmi治疗导致M1巨细胞积累,TNF-α,IL-6,iNOS和Bax的表达增加.

结论:

减弱的S. enterica Typhimurium菌株,特别是DihfABpmi,可以有效治疗癌症.
抗瘤活性通过将瘤微环境重新编程为活性免疫状态来调节,涉及巨细胞透.
向核相关蛋白质基因为开发基于沙门氏菌的癌症免疫疗法提供了可行的策略.

关键词:

这种植物名为S. enterica Typhimurium.这是一种抗癌药物.巨细胞是一个巨细胞.黑色素瘤是一种黑色素瘤.突变物是突变物中的一个.

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