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Updated: Jun 21, 2025

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在大米中使用增强的原始编辑进行高效和精确的基因组删除.

Mengyuan Liu1, Xiang Zhang1, Wen Xu1

  • 1Beijing Key Laboratory of Maize DNA Fingerprinting and Molecular Breeding, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097 China.

aBIOTECH
|July 8, 2024
PubMed
概括

主编辑中介删除 (PDel) 能够在植物中进行精确的基因组修改. 本研究介绍了PDel和PDel/Syn策略,以实现高效准确的基因删除,促进植物研究和作物改进.

关键词:
克里斯普尔是什么意思?克里斯普尔是什么意思?这就是Cas9的情况.精确的基因组删除.主要编辑主要编辑

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Conservative Site-specific Recombination and Phase Variation02:53

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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
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