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在米中进行高效的基因组编辑,使用微型 Cas12f 变体

Zhengyan Ye^1,2, Yuanyan Zhang^1,2, Shiqi He^1,2

¹Sanya Institute of Nanjing Agricultural University, State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Province and Ministry Co-sponsored Collaborative Innovation Center for Modern Crop Production, Nanjing Agricultural University, Nanjing, 210095 China.

|July 8, 2024

概括

增强的CRISPR基因编辑变体,AsCas12f-YHAM和AsCas12f-HKRA,在米中显著提高了效率. AsCas12f-HKRA实现了高达53%的编辑效率,为植物遗传修饰提供了新的工具.

科学领域:

植物分子生物学植物分子生物学
基因组编辑技术的技术.
农业生物技术农业生物技术

背景情况:

虽然CRISPR/Cas9被广泛使用,但它存在一些局限性,例如PAM要求和交付挑战.
来自*Acidibacillus sulfuroxidans*的超紧Cas12f (AsCas12f) 比SpCas9更小,并针对富含T的图案.
在植物中,AsCas12f的编辑效率很低,其变体的性能是未知的.

研究的目的:

为了评估大米中AsCas12f变体的基因组编辑效率.
探索AsCas12f在植物中有针对性的DNA删除方面的潜力.

主要方法:

对大米中AsCas12f变体的基因组裂变活性进行系统研究.
编辑效率和DNA修改模式的分析.

主要成果:

无论是AsCas12f-YHAM还是AsCas12f-HKRA,都显示了在米中更高的编辑效率.
AsCas12f-HKRA实现了高达53%的编辑效率.
AsCas12f 主要诱导删除,具有特定的模式和大小范围 (10-11 bp).

结论:

AsCas12f变种,特别是AsCas12f-HKRA,为植物提供了改进的基因组编辑工具.
AsCas12f独特的删除模式显示了针对性DNA删除应用的潜力.
这些发现扩大了农业精确基因改造的可能性.

关键词:

这就是AsCas12f.这就是CRISPR/CasPR.在Cas12中,Cas12是Cas12中的一个.基因组编辑基因组编辑大米大米大米大米大米

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