LINC01138表达了两种新型异构体,并在质瘤细胞中起到抑制因子的作用
Chao Zhang1, Ao Xu2, Ruoyu Liu3
1Department of Neurosrugery, Tianjin Union Medical Center, Tianjin, 300000, China.
Heliyon
|July 8, 2024
概括
长跨基因非编码RNA 1138 (LINC01138) 在质瘤中被下调,抑制瘤生长. 过度表达LINC01138抑制了质瘤细胞的增殖,迁移和入侵,突出了其抑制瘤的作用.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 质母细胞瘤表现出侵略性的透和具有挑战性的预后.
- 长跨基因非编码RNA 1138 (LINC01138) 最初被归类为一种跨基因非编码RNA.
- 在质母细胞瘤中LINC01138的功能作用在很大程度上仍未被阐明.
研究的目的:
- 调查LINC01138在质母细胞瘤中的功能影响.
- 探索LINC01138在侵袭性瘤透和预后中的作用.
- 为了阐明LINC01138在质瘤中的治疗潜力.
主要方法:
- 使用血素和氨酸 (H&E) 染色进行质瘤分类.
- 定量实时聚合酶链反应 (qRT-PCR) 和凝电泳以评估LINC01138的表达和识别异型.
- 细胞增殖,迁移和入侵测定 (细胞计数,CCK-8,Transwell) 在质瘤细胞系中的LINC01138操纵后.
- 转录组测序以确定LINC01138.8的监管目标.
主要成果:
- 在质瘤组织和细胞系中,LINC01138的下调显著,发现了两种新型异构体.
- 过度表达LINC01138抑制了结质瘤细胞的增殖,迁移和入侵.
- 抑制LINC01138增强了瘤恶性.
- 转录基因组测序显示,LINC01138作为胰岛素类生长因子1 (IGF1) 表达的抑制剂.
结论:
- 在质瘤中,LINC01138异型表现出显著的瘤抑制作用.
- LINC01138显示了作为阻碍质瘤进展的治疗点的潜力.
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