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在Dendrobium huoshanense中的B-box成员的基因组规模识别,表达和进化分析

Hui Deng¹, Yingyu Zhang², Muhammad Aamir Manzoor³

¹Anhui Dabieshan Academy of Traditional Chinese Medicine, Anhui Engineering Research Center for Eco-Agriculture of Traditional Chinese Medicine, College of Biological and Pharmaceutical Engineering, West Anhui University, Luan, 237012, China.

|July 8, 2024

概括

这项研究在Dendrobium huoshanense中确定了23个B-box (BBX) 基因,揭示了它们的核定位和多样化的表达模式,以应对寒冷压力和激素. 这些发现增强了对植物BBX基因家族功能的理解.

科学领域:

植物分子生物学植物分子生物学
基因组学就是基因组学.
生物化学生物化学

背景情况:

B-box (BBX) 蛋白质是植物发育,形态发生和应激反应的关键调节者.
BBX蛋白质影响光形形成,开花时间和生物/无生物应激弹性.

研究的目的:

为了识别和描述Dendrobium huoshanense中的BBX基因家族.
在各种刺激下分析 DhBBX 基因的遗传学关系,基因结构和表达模式.

主要方法:

生物信息分析包括遗传学和对线性分析.
基因结构,保存型号识别和亚细胞局部化预测.
定量逆转录PCR (qRT-PCR) 用于在激素和寒冷压力治疗下表达特征.

主要成果:

在D. huoshanense中确定了23个DhBBX基因,将其分隔成五个家族遗传分类.
所有预测的BBX蛋白都位于核中,具体证实了DhBBX21和DhBBX9.
表达式分析揭示了 DhBBX 基因在激素治疗和寒冷压力反应中的差异调节.

结论:

该研究提供了 DhBBX 基因家族的全面分析,阐明了它们的进化关系和功能角色.
结果提供了BBX基因对D. huoshanense环境和荷尔蒙线索的反应调节机制的见解.
这项工作为进一步研究BBX基因家族在各种植物物种中的功能奠定了基础.

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