纳斯法丁-1减弱脂多糖诱导人类牙纸细胞的炎症反应和衰老
Lili Zhang1, Bo Pang2, Rong Wang1
1Department of Stomatology, Shijingshan Teaching Hospital of Capital Medical University, Beijing Shijingshan Hospital, Beijing, 100043, China.
Heliyon
|July 8, 2024
概括
纳斯法丁-1可以保护人类牙纸细胞免受由脂聚糖 (LPS) 引起的炎症和衰老. 这种由SIRT1调节的可能为牙炎提供一种新的治疗方法.
科学领域:
- 细胞生物学 细胞生物学
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
背景情况:
- 脂聚糖 (LPS) 触发人类牙纸细胞 (hDPC) 的炎症和损伤,导致牙炎.
- 纳斯法-1是一种,在神经元和外围组织中具有已知的作用.
研究的目的:
- 调查纳斯法-1在hDPCs中对LPS诱导的炎症和衰老的新型保护作用.
- 阐明潜在的分子机制,包括SIRT1.1的作用.
主要方法:
- 用LPS和Nesfatin-1治疗hDPCs的治疗方法.
- 测量炎症因子表达的方法.
- 测定端粒酶活性和hTERT和TERF2的表达.
- 与衰老相关的β-galactosidase (SA-β-gal) 的染色.与衰老相关的β-galactosidase (SA-β-gal) 的染色.
- 对PAI-1,p16和SIRT1表达的分析.
- 在SIRT1基因沉默实验中.
主要成果:
- 纳斯法-1显著抑制了LPS诱导的炎症因素.
- 纳斯法廷-1恢复了端粒酶活性和hTERT和TERF2的表达.
- 纳斯法廷-1减弱了LPS诱导的细胞衰老,减少了SA-β-gal染色,PAI-1和p16的表达.
- 纳斯法丁-1 挽救了LPS诱导的SIRT1.1下调.
- 静止SIRT1消除了Nesfatin-1对衰老的保护作用.
结论:
- 纳斯法-1在hDPCs中表现出对LPS诱导的炎症和衰老的保护性.
- 纳斯法-1的保护机制涉及SIRT1.1的调节.
- 涅斯法-1具有作为治疗牙炎的治疗剂的潜力.
相关概念视频
Inflammation
Overview
The Effect of Aging on Tissues
Several body functions deteriorate with age. The external signs of aging are easily identifiable. For example, the skin becomes dry, less elastic, and thins out, forming wrinkles. The skin of the face begins to appear looser due to a decrease in the levels of elastic and collagen fibers in the connective tissue. Additionally, melanin production in the hair follicle decreases with age, resulting in gray hair. Moreover, the senses of sight and hearing decline, so glasses and hearing aids may...
Psychoneuroimmunology: Diabetes and Cancer
Chronic stress has been linked to both the onset and progression of serious health conditions, including Type 2 diabetes and cancer. Type 2 diabetes, a widespread chronic illness, is closely associated with obesity and insulin resistance, both of which often worsen under stress. Studies indicate that men experiencing high levels of chronic stress face a 45% higher risk of developing diabetes compared to those with minimal stress. Stress triggers physiological responses that elevate blood...
The Oral Microbiota
The oral microbiome includes a complex ecosystem comprising over 700 microbial species, identified through genomic sequencing and culture-based analyses to date. This community includes a core microbiome, found universally among individuals, and a variable component influenced by environmental factors such as diet, lifestyle, and host genetics. Site-specific conditions, including oxygen gradients, pH levels, and nutrient availability, determine the spatial distribution of these microorganisms...
Diabetic Neuropathy
DefinitionDiabetic neuropathy is nerve damage caused by long-standing diabetes mellitus. It results directly from prolonged high blood sugar levels.PathophysiologyThe pathophysiology of diabetic neuropathy involves both metabolic and vascular disturbances triggered by chronic hyperglycemia.Metabolic injury: Elevated glucose levels activate the polyol pathway within nerve cells, leading to the accumulation of sorbitol and fructose. This increases oxidative stress, disrupts normal nerve...


