来自Bacillus sp. 的性角酶. DRS4有效地生物降解羽,以合成改进的质/细菌纳米纤维素基生物塑料
Tiruwork Zewudie Admasie1, Fantahun Biadglegne2,3, Ebrahim M Abda1,4
1Biotechnology Department, Addis Ababa Science and Technology University, Addis Ababa, P.O.Box 1647, Ethiopia.
Heliyon
|July 8, 2024
概括
羽可以用一种新型细菌,Bacillus sp.转化为有价值的生物塑料. DRS4.4 这是一个很好的方法. 这种细菌有效地降解羽毛,产生富含蛋白质的水解剂,当与细菌纳米纤维素相结合时,产生强大,耐热的生物塑料.
科学领域:
- 生物技术是生物技术.
- 材料科学 材料科学 材料科学
- 微生物学 微生物学
背景情况:
- 羽是一种丰富的,可持续的废物.
- 农业副产品的价值化对于循环经济至关重要.
- 酶降解为加工质材料提供了一种绿色方法.
研究的目的:
- 为了隔离和表征一种用于羽退化的新型角质溶解细菌.
- 为了优化酶的生产,以实现高效的羽毛水解.
- 为了合成和评估一种新型的生物塑料增强细菌纳米纤维素从羽毛水解.
主要方法:
- 隔离和识别一个溶性细菌 (Bacillus sp. DRS4) 使用16S rRNA基因测序.
- 优化物理化学参数以提高酶产量.
- 羽的酶性消化和蛋白质水解剂的表征.
- 用细菌纳米纤维素 (BNC) 增强的生物塑料的合成.
- 评估机械 (拉力强度) 和热 (化温度) 性能,并进行FTIR分析.
主要成果:
- 巴西勒斯种类 (Bacillus sp.) 的存在 DRS4在48小时内有效降解了羽.
- 在优化条件下,酶产量增加了30%.
- 羽毛水解剂含有251.145毫克/毫升总蛋白质.
- 由此产生的生物塑料具有5.769MPa的抗拉强度和127.5°C的化温度.
- FTIR分析证实了由于BNC增强而增强的性能.
结论:
- 巴西勒斯种类 (Bacillus sp.) 的存在 DRS4是一种高效的菌株,用于性角酶的产生.
- 羽水解剂与BNC结合,形成了一个有前途的生物塑料材料.
- 这一过程提供了一条可持续的途径,将羽废弃物升级为增值产品.
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