自动生物技术 - - 一个用于自动化菌株工程的多功能生物发明厂
Tobias Michael Rosch1, Julia Tenhaef1, Tim Stoltmann1
1Institute of Bio- and Geosciences, IBG-1: Biotechnology, Forschungszentrum Jülich, D-52425 Jülich, Germany.
ACS synthetic biology
|July 8, 2024
概括
自动生物技术平台为微生物菌株工程提供全自动化解决方案,加速开发可再生替代品的新型生产者菌株. 该系统增强了Gram负和Gram阳性细菌的生物基础能力.
科学领域:
- 生物技术和合成生物学
- 微生物工程是微生物的工程.
- 在生命科学领域的自动化
背景情况:
- 向可再生资源的转变需要先进的微生物生产菌株.
- 手动微生物工程方法不足以满足日益增长的需求.
- 生物发明公司正在出现,通过自动化解决这些挑战.
研究的目的:
- 引入AutoBioTech平台,这是一个完全自动化的微生物菌株构造系统.
- 为了证明该平台在自动基因组编辑和转换方面的能力.
- 为了使高通量菌株工程能够对格拉姆阴性和格拉姆阳性细菌进行.
主要方法:
- 开发一个14个设备的自动化实验室系统 (AutoBioTech).
- 实现模块化工作流程用于自动化微生物转换.
- 集成CRISPR/Cas9工具箱用于自动基因组编辑.
- 建立对大肠杆菌和谷氨酸菌的自动化转化协议.
主要成果:
- 自动生物技术 (AutoBioTech) 平台成功地在没有人类干预的情况下实现了自动化菌株构造.
- 通过模块化克隆和CRISPR/Cas9实现了大肠杆菌的自动转化.
- 通过电穿孔建立了强大的*C. glutamicum*的自动化转化.
- 该平台展示了多功能性和模块化工作流程之间的无过渡.
结论:
- 自动生物技术平台显著加速了微生物菌株工程.
- 它的模块化和自动化支持格拉姆阴性和格拉姆阳性细菌.
- 这一系统提升了生物基础设施开发新型微生物生产者的能力.
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