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Synthetic Biology02:55

Synthetic Biology

5.8K
Synthetic biology is an interdisciplinary science that involves using principles from disciplines such as engineering, molecular biology, cell biology, and systems biology. It involves remodeling existing organisms from nature or constructing completely new synthetic organisms for applications such as protein or enzyme production, bioremediation, value-added macromolecule production, and the addition of desirable traits to crops, to name a few.
Golden rice
Golden rice is a genetically modified...
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Bioreactor Design and Operational System01:29

Bioreactor Design and Operational System

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Bioreactors are engineered vessels designed to cultivate microorganisms under controlled conditions for industrial bioprocessing. They maintain sterility and allow precise regulation of pH, temperature, oxygen, and nutrient levels to optimize microbial growth and metabolite production. Bioreactors range from small laboratory units of 1 liter to industrial systems holding up to 500,000 liters, though only about 75% of their volume is actively used for fermentation. The remaining headspace...
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Bioreactor Controls-III01:22

Bioreactor Controls-III

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Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...
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Designing Growth Media for Bioreactors01:30

Designing Growth Media for Bioreactors

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Growth media provide essential nutrients that support cell growth and metabolism, thereby enhancing the yield of valuable products such as enzymes, antibiotics, and biomass. Designing an effective growth medium involves balancing all components to prevent nutrient limitations or toxic excesses, both of which can impair growth and reduce product yields.Composition of a Typical Growth MediumA typical growth medium contains carbon and nitrogen sources, salts, vitamins, trace elements, and...
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Scale-Up Processes01:14

Scale-Up Processes

88
The scale-up of microbial fermentation processes is essential in industrial biotechnology, allowing the transition from laboratory-scale experiments to commercial-scale production while aiming to maintain product yield and quality. This process requires meticulous adjustment of equipment design, process parameters, and contamination control strategies to accommodate increasing culture volumes.At the laboratory scale, cultures are typically maintained in 1 to 10-liter glass or autoclavable...
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Upstream Processing01:27

Upstream Processing

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Upstream processing represents a critical phase in biomanufacturing, wherein biological systems such as microorganisms, mammalian cells, or insect cells are cultivated to produce therapeutic proteins, vaccines, enzymes, or other biologically derived products. This phase encompasses all steps from the selection and genetic manipulation of the production organism to the cultivation of cells in bioreactors under tightly controlled environmental conditions.Host Selection and Genetic OptimizationThe...
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A High-throughput Automated Platform for the Development of Manufacturing Cell Lines for Protein Therapeutics
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自动生物技术 - - 一个用于自动化菌株工程的多功能生物发明厂.

Tobias Michael Rosch1, Julia Tenhaef1, Tim Stoltmann1

  • 1Institute of Bio- and Geosciences, IBG-1: Biotechnology, Forschungszentrum Jülich, D-52425 Jülich, Germany.

ACS synthetic biology
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概括

自动生物技术平台为微生物菌株工程提供全自动化解决方案,加速开发可再生替代品的新型生产者菌株. 该系统增强了Gram负和Gram阳性细菌的生物基础能力.

关键词:
这就是CRISPR/Cas9的作用.在Corynebacterium glutamicum的研究中,我们发现埃舍里希亚大肠杆菌 (Escherichia coli) 是一个大肠杆菌.自动化自动化自动化自动化模块化克隆是一种模块化克隆.

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科学领域:

  • 生物技术和合成生物学
  • 微生物工程是微生物的工程.
  • 在生命科学领域的自动化

背景情况:

  • 向可再生资源的转变需要先进的微生物生产菌株.
  • 手动微生物工程方法不足以满足日益增长的需求.
  • 生物发明公司正在出现,通过自动化解决这些挑战.

研究的目的:

  • 引入AutoBioTech平台,这是一个完全自动化的微生物菌株构造系统.
  • 为了证明该平台在自动基因组编辑和转换方面的能力.
  • 为了使高通量菌株工程能够对格拉姆阴性和格拉姆阳性细菌进行.

主要方法:

  • 开发一个14个设备的自动化实验室系统 (AutoBioTech).
  • 实现模块化工作流程用于自动化微生物转换.
  • 集成CRISPR/Cas9工具箱用于自动基因组编辑.
  • 建立对大肠杆菌和谷氨酸菌的自动化转化协议.

主要成果:

  • 自动生物技术 (AutoBioTech) 平台成功地在没有人类干预的情况下实现了自动化菌株构造.
  • 通过模块化克隆和CRISPR/Cas9实现了大肠杆菌的自动转化.
  • 通过电穿孔建立了强大的*C. glutamicum*的自动化转化.
  • 该平台展示了多功能性和模块化工作流程之间的无过渡.

结论:

  • 自动生物技术平台显著加速了微生物菌株工程.
  • 它的模块化和自动化支持格拉姆阴性和格拉姆阳性细菌.
  • 这一系统提升了生物基础设施开发新型微生物生产者的能力.