工程大肠杆菌以高效生产具有低分子质量和粘度的结肠酸
Jiaxin Wu1, Ming Huang1, He Liu1
1School of Biotechnology and Key Laboratory of Industrial Biotechnology of Ministry of Education, Jiangnan University, Wuxi 214122, China.
Journal of agricultural and food chemistry
|July 8, 2024
概括
经过工程 *Escherichia coli* 产生更高产量的酸 (CA),一种多用途的生物聚合物. 改性菌株实现了显著增加的CA产量,并为食品和医疗保健应用量身定制的分子质量和粘度.
科学领域:
- 微生物学 微生物学
- 生物技术是生物技术.
- 聚合物科学 聚合物科学
背景情况:
- 酸 (CA) 是一种多用途的外聚糖,在食品和医疗保健行业具有显著的潜力.
- 之前的工作确立了*Escherichia coli*菌株WWM16,用于高效的CA生产.
研究的目的:
- 为了工程 *Escherichia coli* WWM16 增强CA的生产,减少分子质量和粘度.
- 用于工业应用的工程菌株生产的AC的特性.
主要方法:
- 通过删除 *mcbR*, *opgD*, *opgG* 和 *opgH* 的基因来创建 *E. coli* WWM16 的基因工程,从而创建了菌株 WWM166.
- 在菌株WWM166/pWpD1.1中使用CRISPR干扰 (CRISPRi) 下调聚合酶基因*wcaD*.
- 净化和表征CA,包括分子质量,粘度,流动性,高度和抗氧化活性.
主要成果:
- 菌株WWM166产生了18.1g/L的CA.
- 菌株WWM166/pWpD1实现了 49.9 g/L CA 的产量,其分子质量较低.
- 对比分析显示了基于分子质量的CA性质的差异.
结论:
- 基因改造和CRISPRi介导的基因表达控制有效地提高了CA产量,并调节了它在大肠杆菌中的特性.
- 具有特定分子质量的定制CA显示出在食品和化品行业内延长寿命和皮肤保护方面的应用潜力.
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