来自脂肪组织衍生的干细胞的细胞外囊泡以依赖脚手架的方式刺激血管生成
V E Getova1,2, E Orozco-García1,2,3, S Palmers1
1Department of Pathology and Medical Biology, University Medical Center Groningen, University of Groningen, Hanzeplein 1 (EA11), 9713 GZ, Groningen, The Netherlands.
Tissue engineering and regenerative medicine
|July 8, 2024
概括
脂肪组织衍生的树皮细胞外细胞囊泡 (ASC-EVs) 促进血管网络的形成. 在ASC-EV中丰富的亲血管性miRNA,特别是miR-1290,显示出再生疗法的潜力.
科学领域:
- 再生医学是一种再生医学.
- 细胞生物学 细胞生物学
- 生物材料是一种生物材料.
背景情况:
- 脂肪组织衍生体细胞 (ASC) 分泌细胞外囊泡 (EVs),调节组织再生.
- ASC-EVs在内皮细胞功能和血管化中的作用在很大程度上仍未被探索.
- 了解ASC-EVs的益血管性潜力对于开发新型治疗策略至关重要.
研究的目的:
- 研究ASC-EVs对内皮细胞的亲血管效应.
- 为了识别ASC-EV中促进血管化的miRNA载荷.
- 探索ASC-EV在组织工程支架中用于血管再生的应用.
主要方法:
- 从正常性和低氧性ASC培养物中分离EVs.
- 使用miRseq进行miRNA分析,并对EV标记物进行表征.
- 在水凝支架中使用ex vivo大动脉环测定来评估ASC-EVs的益血管性活性.
主要成果:
- 在大动脉环模型中,ASC-EV显著增强了血管网络的形成.
- 血管网络的拓和稳定性取决于所使用的水凝支架.
- ASC-EVs被丰富在特定的miRNA家族 (Let-7,miR-23/27/24) 和显著的miR-1290.
结论:
- ASC-EVs促进体外和体外血管化,由像miR-1290.0.这样的亲血管性miRNA驱动.
- 这些发现凸显了ASC-EVs在再生和再血管化应用中的治疗潜力.
- 装有ASC-EV的ECM水凝为未来的组织工程疗法提供了一个有希望的平台.
更多相关视频
11:00Isolation and Enrichment of Human Adipose-derived Stromal Cells for Enhanced Osteogenesis
Published on: January 12, 2015
11.5K
06:56Author Spotlight: The Significance of Isolation, Culture, and Adipogenic Induction of SVF-Derived Preadipocytes from Mouse Perivascular Adipose Tissue
Published on: July 21, 2023
2.1K
相关概念视频
01:10Mechanism of Angiogenesis
5.4K
Blood vessel formation starts early during embryonic development, around day 7. In the extraembryonic yolk sac, mesodermal precursor cells called hemangioblast proliferate and differentiate into angioblast. Angioblasts express vascular endothelial growth factor receptor 2 or VEGFR2, which binds VEGF-A, a proangiogenic factor, guiding blood vessel formation. VEGF signaling promotes angioblasts to form a blood island in the developing embryo. Angioblasts further differentiate, giving rise to...
5.4K
01:24Regulation of Angiogenesis and Blood Supply
2.5K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
2.5K