对产生碳酸酶酶的细菌进行研究,用于CaCO3沉和土壤生物凝固
Wilson Mwandira1, Maria Mavroulidou2, Martijn Timmermans3
1Division of CIBSE, School of the Built Environment and Architecture, London South Bank University, London, UK.
概括
这项研究使用碳酸 anhydrase (CA) 生产细菌沉石,增强土壤性能,用于工程应用. 生物水泥化显著改善了土壤的强度和稳定性,显示了基础设施开发的潜力.
科学领域:
- 地质技术工程 地质技术工程
- 微生物学 微生物学
- 生物技术是生物技术.
背景情况:
- 土壤稳定对于基础设施发展至关重要.
- 生物水泥化为传统方法提供了一个可持续的替代方案.
- 产生碳酸 anhydrase (CA) 的细菌可以诱导石沉以改善土壤.
研究的目的:
- 调查土壤生物凝固的本土CA产生细菌的使用.
- 为了优化处理条件,增强石沉和土壤特性.
- 评估细菌CA在改善土壤工程特性方面的有效性.
主要方法:
- 从铁路堤防土壤中分离和识别CA产生细菌.
- 通过改变pH值,温度,辅因子和二碳酸盐度来优化细菌生长和CA活性.
- 使用SEM,FT-IR和拉曼光谱学对生物沉物的表征.
- 在细粒度土壤中注射预培养细菌和凝固溶液.
主要成果:
- 他们发现了三种具有高CA活性的细菌菌株 (Bacillus licheniformis,Bacillus toyonensis,Bacillus pumilus).
- SEM,FT-IR和拉曼分析证实了石和瓦特里特晶体的形成.
- 经过处理的土壤显示了高达1MPa的不受限制的压力强度增加,碳酸含量增加了2.78%.
- 在水浸浸后提高土壤稳定性证明了成功的生物凝固.
结论:
- 本土的CA产生细菌有效地诱导石沉用于土壤生物凝固.
- 优化的治疗条件增强了细菌CA活动和生物沉物形成.
- 细菌CA介导的土壤稳定显示了改善土壤工程性质和基础设施弹性的巨大潜力.
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