Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Glucose Homeostasis: Pancreatic Islets and Insulin Secretion01:27

Glucose Homeostasis: Pancreatic Islets and Insulin Secretion

1.2K
The pancreatic islets comprising only 1%-2% of the volume are highly vascularized and innervated mini-organs. They contain five endocrine cell types, including β cells that secrete insulin, which is synthesized as a single polypeptide chain, preproinsulin, processed to proinsulin, and finally to insulin and C-peptide. This process is complex and regulated, involving the Golgi complex, the endoplasmic reticulum, and the secretory granules of the β cell.
Insulin and C-peptide are...
1.2K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

A F<sub>420</sub>-dependent Single Domain Chemogenetic Tool for Protein De-dimerization.

Journal of molecular biology·2025
Same author

Automated Plate Reader-Based Assays of Light-Activated GPCRs.

Methods in molecular biology (Clifton, N.J.)·2024
Same author

Modular Light-Emitting Diode Shelving Systems for Scalable Optogenetics.

Methods in molecular biology (Clifton, N.J.)·2024
Same author

Optimized design and in vivo application of optogenetically functionalized Drosophila dopamine receptors.

Nature communications·2023
Same author

CDK6 activity in a recurring convergent kinase network motif.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2023
Same author

Structure-guided optimization of light-activated chimeric G-protein-coupled receptors.

Structure (London, England : 1993)·2022

相关实验视频

Updated: Jun 21, 2025

Author Spotlight: Investigating Islet Abnormalities and Function with a Pseudoislet Protocol
08:04

Author Spotlight: Investigating Islet Abnormalities and Function with a Pseudoislet Protocol

Published on: November 3, 2023

1.9K

胰腺小岛的光遗传学:执行者和影响

Christina G Gangemi1,2,3, Harald Janovjak1

  • 1Flinders Health and Medical Research Institute, College of Medicine and Public Health, Flinders University, Bedford Park, South Australia, Australia.

Diabetes
|July 8, 2024
PubMed
概括

光遗传学可以精确控制胰腺小岛细胞,进步我们对荷尔蒙分泌和葡萄糖平衡的理解. 这项技术对于探索糖尿病机制和潜在治疗方法至关重要.

更多相关视频

Lentiviral Mediated Gene Silencing in Human Pseudoislet Prepared in Low Attachment Plates
11:24

Lentiviral Mediated Gene Silencing in Human Pseudoislet Prepared in Low Attachment Plates

Published on: May 14, 2019

5.4K
A Method for Mouse Pancreatic Islet Isolation and Intracellular cAMP Determination
12:33

A Method for Mouse Pancreatic Islet Isolation and Intracellular cAMP Determination

Published on: June 25, 2014

65.2K

相关实验视频

Last Updated: Jun 21, 2025

Author Spotlight: Investigating Islet Abnormalities and Function with a Pseudoislet Protocol
08:04

Author Spotlight: Investigating Islet Abnormalities and Function with a Pseudoislet Protocol

Published on: November 3, 2023

1.9K
Lentiviral Mediated Gene Silencing in Human Pseudoislet Prepared in Low Attachment Plates
11:24

Lentiviral Mediated Gene Silencing in Human Pseudoislet Prepared in Low Attachment Plates

Published on: May 14, 2019

5.4K
A Method for Mouse Pancreatic Islet Isolation and Intracellular cAMP Determination
12:33

A Method for Mouse Pancreatic Islet Isolation and Intracellular cAMP Determination

Published on: June 25, 2014

65.2K

科学领域:

  • 内分泌学 在内分泌学.
  • 分子生物学分子生物学
  • 细胞生物学 细胞生物学

背景情况:

  • 兰格汉斯小岛是胰腺中重要的内分泌微器官,通过激素分泌 (例如胰岛素,葡萄糖) 调节血糖.
  • 岛屿细胞功能涉及复杂的分子动力学,它们的功能障碍与糖尿病有关.
  • 光遗传学,使用光敏感执行器,可以精确操纵细胞功能.

研究的目的:

  • 审查光遗传学在岛屿细胞生物学中的应用.
  • 讨论光遗传驱动器及其对小岛功能和糖尿病研究的影响.
  • 突出新兴研究对膜调节和岛屿内的细胞内信号.

主要方法:

  • 利用自然光受体蛋白和工程变体进行光遗传学操纵.
  • 将光遗传工具应用于小鼠和人类小岛细胞.
  • 在小岛研究中审查现有的光遗传学文献.

主要成果:

  • 光遗传学允许对岛屿细胞活动进行时空控制,包括激素的产生和分泌.
  • 新兴的研究探索光遗传学研究细胞间通信和信号通路在小岛.
  • 光遗传学方法为研究糖尿病的病理生理学提供了新的途径.

结论:

  • 光遗传学是剖析小岛细胞功能及其在代谢疾病中的作用的强大工具.
  • 对光遗传学执行器的持续开发将提高我们对葡萄糖平衡的理解.
  • 这项技术对未来糖尿病治疗策略具有前景.