通过望远镜检测一种prenyltransferase和一种diterpene synthase,将非自然的FPP衍生物转化为diterpenoids
Henry Struwe1, Heng Li2, Finn Schrödter1
1Institute of Organic Chemistry, Leibniz University Hannover, Schneiderberg 1B, 30167 Hannover, Germany.
Organic letters
|July 8, 2024
概括
研究人员使用酶和非天然基质制造了新的二类物质. 这种生物转化方法产生了新的环多德干核和宏环,揭示了独特的1,12-烯循环.
科学领域:
- 自然产品的合成自然产品的合成
- 酶式生物转化 酶式生物转化
- 有机化学 有机化学
背景情况:
- 迪特尔类动物是复杂的自然产物,具有多样化的生物活动.
- 酶合成为复杂分子提供了一条可持续的途径.
- 格拉尼尔格拉尼尔酸盐合成酶 (GGPPS) 和斯帕塔-13,17-二烯合成酶 (SpS) 是基生物合成中的关键酶.
研究的目的:
- 探索使用非天然基质的新型特类的酶合成.
- 为了研究GGPPS和SpS的循环化能力与修改的FPP衍生品.
- 描述新的生物转化产品和反应机制.
主要方法:
- 作为基质使用非天然的Farnesyl Pyrophosphate (FPP) 衍生物.
- 采用一种酶的延长循环级联,其中包括来自*Streptomyces cyaneofuscatus*的GGPPS和来自*Streptomyces xinghaiensis*的SpS.
- 使用光谱学方法对由此产生的生物转化产品进行了表征.
主要成果:
- 成功合成了四种新的生物转化产品.
- 确定了三种新型的循环多德干核和一个宏环乙烯.
- 观察到一个以前未报告的1,12-烯循环,形成一个非结合的1,4-diene.
结论:
- 使用工程基质的酶延长-循环级联对于新型的迪特尔类合成是有效的.
- 这项研究扩大了已知烯循环反应的范围.
- 这种方法为进一步研究提供了独特的分子支架.
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