藻类涂层的氧化铜纳米颗粒的合成,在养殖中具有潜在的应用
Negar Nasri1, Hajar-Alsadat Mansouri-Tehrani1, Ghasem Dini2
1Department of Biotechnology, Faculty of Biological Science and Technology, University of Isfahan, Isfahan, 81746-73441, Iran.
Fish & shellfish immunology
|July 8, 2024
概括
覆盖藻类的氧化铜纳米颗粒 (CuO NPs) 显示出对抗Vibrio harveyi的增强抗菌活性,并改善了细胞活力. 没有涂层的CuONP显示出优越的抗氧化特性.
科学领域:
- 材料科学 材料科学 材料科学
- 纳米技术纳米技术
- 环境科学 环境科学
背景情况:
- 铜 (Cu) 对于生物功能至关重要.
- 氧化铜纳米颗粒 (CuO NPs) 是为了各种应用而合成的.
- 海藻提取物为纳米粒子功能化提供了潜力.
研究的目的:
- 为了合成和表征藻类涂层CuONP.
- 评估涂层和未涂层CuONP的抗菌和抗氧化活性.
- 评估CuO NPs在血细胞和人类淋巴细胞上的细胞毒性.
主要方法:
- 用于CuO NP合成的沉方法.
- 用 Sargassum angustifolium 藻类提取物涂覆 CuO NPs.
- 抗菌检测对抗Vibrio harveyi. 的抗菌检测.
- 使用IC50.0.使用抗氧化剂活性评估.
- 对类血细胞和人类淋巴细胞的细胞毒性评估.
主要成果:
- 与未涂层的NP (MIC 1000 μg/mL) 相比,涂层的CuO NP表现出增强的抗菌活性 (MIC 500 μg/mL).
- 没有涂层的CuONP显示出优异的抗氧化活性 (IC50 ≥16μg/mL).
- 涂层CuONP的低度 (10μg/ml) 提高了的血细胞活力,而涂层和未涂层NP的高度降低了活力.
结论:
- Sargassum angustifolium藻类提取物增强了CuO NPs的抗菌功效.
- 藻类覆盖的CuONP显示出水产养殖应用的潜力,具有更好的生物相容性.
- 这项研究提供了CuO NP在细胞上的第一个定量毒性评估,使得与人类细胞进行比较分析.
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