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相关概念视频

Titration of a Weak Base with a Strong Acid01:20

Titration of a Weak Base with a Strong Acid

4.7K
The titration curve of a weak base like ammonia with a strong acid like hydrochloric acid is the mirror image of the titration curve of a weak acid with a strong base.
Using the ICE table and substituting the Kb value, we calculate the initial pH of 50 mL of 0.1 M ammonia to be 11.11. Addition of 25 mL of 0.1 M hydrochloric acid to this solution of ammonia results in a buffer with an equal concentration of ammonia and ammonium ions. The pH of this buffer can be calculated by substituting these...
4.7K
Titration of a Weak Acid with a Strong Base01:30

Titration of a Weak Acid with a Strong Base

2.2K
In titrating a weak acid with a strong base, different calculation methods are applied at various stages. Initially, the pH of a weak acid like acetic acid is calculated using its dissociation constant (Ka) and an ICE table. Upon addition of a strong base such as sodium hydroxide, a buffer forms, and its pH is determined using the Henderson-Hasselbalch equation. As more base is added and the titration reaches the halfway point, the pH becomes equal to the pKa of the acid, indicating equal...
2.2K
Titration Calculations: Weak Acid - Strong Base03:55

Titration Calculations: Weak Acid - Strong Base

44.2K
Calculating pH for Titration Solutions: Weak Acid/Strong Base
For the titration of 25.00 mL of 0.100 M CH3CO2H with 0.100 M NaOH, the reaction can be represented as:
44.2K
Instrument Calibration01:12

Instrument Calibration

170
Instrument calibration is essential for ensuring that instruments produce accurate and consistent results. It is vital in manufacturing, healthcare, testing laboratories, and scientific research. Calibration processes are specific to each instrument and help enhance data accuracy. Each instrument has a unique calibration process tailored to its design and function to improve data accuracy.
Analytical Balance Calibration
An analytical balance measures mass and requires regular calibration to...
170
Titration of a Weak Acid with a Weak Base01:08

Titration of a Weak Acid with a Weak Base

2.8K
Weak acids and bases do not undergo dissociation completely, and titrations between these two are rarely studied. When such studies are performed, say, for the titration of a weak acid with a weak base, the titration curve plots the change in pH as a function of the volume of base added. Take the titration of acetic acid with ammonia, for instance. During the titration, these two species form ammonium acetate and water, but the pH change is slow and gradual.
As a result, there is no simple...
2.8K
Titration Calculations: Strong Acid - Strong Base02:28

Titration Calculations: Strong Acid - Strong Base

29.5K
Calculating pH for Titration Solutions: Strong Acid/Strong Base
A titration is carried out for 25.00 mL of 0.100 M HCl (strong acid) with 0.100 M of a strong base NaOH. The pH at different volumes of added base solution can be calculated as follows:
(a) Titrant volume = 0 mL. The solution pH is due to the acid ionization of HCl. Because this is a strong acid, the ionization is complete and the hydronium ion molarity is 0.100 M. The pH of the solution is then:
29.5K

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相关实验视频

Updated: Jun 21, 2025

Measuring Enzymatic Stability by Isothermal Titration Calorimetry
08:37

Measuring Enzymatic Stability by Isothermal Titration Calorimetry

Published on: March 26, 2019

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校准ITC仪器:弱基中和问题

Žiga Medoš1, Marija Bešter-Rogač1, Epameinondas Leontidis2

  • 1Faculty of Chemistry and Chemical Technology, University of Ljubljana, Večna pot 113, 1000, Ljubljana, Slovenia.

Analytical biochemistry
|July 8, 2024
PubMed
概括
此摘要是机器生成的。

精确的异热定位热量计 (ITC) 校准需要考虑定位器体积损失,特别是在精确的测量中. 一个可变体积的协议有助于纠正从注射器尖端的扩散性HCl损失,提高热因子的准确性.

关键词:
酸反应是一种酸反应.校准 校准 校准 校准 校准 校准 校准热量测量方法热量测量方法扩散扩散是一种扩散.异热量定位热量计 异热量定位热量计

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Isothermal Titration Calorimetry for Measuring Macromolecule-Ligand Affinity
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Isothermal Titration Calorimetry for Measuring Macromolecule-Ligand Affinity

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Optimization of a Quantitative Micro-neutralization Assay
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Optimization of a Quantitative Micro-neutralization Assay

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相关实验视频

Last Updated: Jun 21, 2025

Measuring Enzymatic Stability by Isothermal Titration Calorimetry
08:37

Measuring Enzymatic Stability by Isothermal Titration Calorimetry

Published on: March 26, 2019

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Isothermal Titration Calorimetry for Measuring Macromolecule-Ligand Affinity
08:45

Isothermal Titration Calorimetry for Measuring Macromolecule-Ligand Affinity

Published on: September 7, 2011

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Optimization of a Quantitative Micro-neutralization Assay
10:09

Optimization of a Quantitative Micro-neutralization Assay

Published on: December 14, 2016

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科学领域:

  • 分析化学 分析化学
  • 生物物理化学 生物物理化学

背景情况:

  • 同热度定位热度计 (ITC) 仪器提供高精度,但需要化学校准才能准确.
  • 盐酸 (HCl) 与弱基TRIS的反应是确定热因子的标准方法.

研究的目的:

  • 用现代ITC仪器调查校准问题,特别关注HCl/TRIS反应.
  • 识别和量化影响ITC测量的精度的错误来源.

主要方法:

  • 使用VP-ITC和两个纳米ITC进行校准实验.
  • 采用了可变标称体积协议来检测和纠正标称损失.
  • 进行了数学建模,以分析从注射器尖端散发性丢失的HCl.
  • 从复制数据中估计的数据方差函数,以改进最小正方形的拟合.

主要成果:

  • 观察到显著的标量体积不足 (Δv ≈ 0.3 μL),归因于从注射器尖端的HCl扩散.
  • 证明了可变体积协议在弥补这一缺口方面的有效性.
  • 发现碳酸盐污染和弱基解可以引入实质性错误.
  • 在不同的仪器中量化低信号精度 (σ ≈ 1 μJ) 和标称体积不确定性.

结论:

  • 标称器的扩散损失是影响ITC准确性的关键因素,需要使用可变体积协议进行精确的校准.
  • 标准校准协议可能不足,因为未解决的错误来源,如扩散,污染和水解.
  • 准确确定热因子需要仔细考虑这些系统错误和仪器特定的不确定性.