相关实验视频

Updated: Jun 21, 2025

Labelling and Visualization of Mitochondrial Genome Expression Products in Baker's Yeast Saccharomyces cerevisiae

Labelling and Visualization of Mitochondrial Genome Expression Products in Baker's Yeast Saccharomyces cerevisiae

Published on: April 11, 2021

在Saccharum属中的三个基础物种的基于图形的线粒体基因组

Sicheng Li^1,2, Zhen Wang^1,2, Yanfen Jing³

¹State Key Laboratory of Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning, 530004, China.

Plant cell reports

|July 8, 2024

概括

这项研究介绍了三种关键甘物种的第一个基于图形的线粒体基因组,揭示了广泛的变异和进化见解. 这些发现增强了我们对甘基因组学和进化论的理解.

科学领域:

基因组学就是基因组学.
植物生物学植物生物学
进化生物学进化生物学

背景情况:

甘 (Saccharum) 是一个重要的全球作物,对糖生产作出了重大贡献.
了解甘的遗传多样性和进化历史对于作物改进至关重要.
线粒体基因组在植物细胞功能和进化中起着关键作用.

研究的目的:

组装和描述三种基础的Saccharum物种的完整线粒体基因组.
研究Saccharum线粒体基因组内的泛结构变异和进化过程.
阐明Saccharum属中主要的跨种谱系的进化关系和历史.

主要方法:

使用Illumina和牛津纳米孔技术进行测序.
15个完整的线粒体基因组的基于图的组合.
对比基因组学和遗传学分析.
重复介导的重组的PCR验证.

主要成果:

在S.spontaneum,S.robustum和S. officinarum中组合了八种不同的线粒体基因组类型.
在所有线粒基因组中确定了51个独特的基因 (32个蛋白质编码,3个rRNA,16个tRNA).
在S. officinarum和S. robustum中证实了长时间和短时间的重复介导的重组.
遗传学分析表明S. officinarum和S. robustum的不同进化路径.
杂交的假设涉及S. officinarum加入,有助于现代种植的甘变异.

结论:

这项研究为三种关键的Saccharum物种提供了第一个基于图的线粒体基因组.
在Saccharum线粒体基因组中发现了显著的泛结构变异和进化过程.
这些发现提供了关于甘血统的进化历史和关系的见解,为未来的育种策略提供了信息.

关键词:

圣体 (Saccharum) 是一个圣体.线粒体基因组的基因组人类遗传学学.通过重复介导的重组组合.

更多相关视频

Using Phylogenetic Analysis to Investigate Eukaryotic Gene Origin

Using Phylogenetic Analysis to Investigate Eukaryotic Gene Origin

Published on: August 14, 2018

Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing

Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing

Published on: July 28, 2017

相关概念视频

Evolutionary Relationships through Genome Comparisons

Evolutionary Relationships through Genome Comparisons

Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...

Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes

Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes

The present-day mitochondrial and chloroplast genomes have retained some of the characteristics of their ancestral prokaryotes and also have acquired new attributes during their evolution within eukaryotic cells. Like prokaryotic genomes, mitochondrial and chloroplast genomes neither bind with histone-like proteins nor show complex packaging into chromosome-like structures, as observed in eukaryotes. Unlike mitotic cell divisions observed in eukaryotic cells, mitochondria and chloroplasts...

Animal Mitochondrial Genetics

Animal Mitochondrial Genetics

Among all the organelles in an animal cell, only mitochondria have their own independent genomes. Animal mitochondrial DNA is a double-stranded, closed-circular molecule with around 20,000 base pairs. Mitochondrial DNA is unique in that one of its two strands, the heavy, or H, -strand is guanine rich, whereas the complementary strand is cytosine rich and called the light, or L, -strand. Compared to nuclear DNA, mitochondrial DNA has a very low percentage of non-coding regions and is marked by...