相关实验视频
Updated: Jun 21, 2025

09:39
Modeling Myotonic Dystrophy 1 in C2C12 Myoblast Cells
Published on: July 29, 2016
15.3K
细胞外C1qbp通过抑制NFATc1来抑制肌体发生
Jin-Man Kim1, Ho Kyoung Kim1, Han Jin Cho1
1Asan Institute for Life Sciences, Asan Medical Center, Seoul, 05505, Republic of Korea.
Scientific reports
|July 8, 2024
概括
新的研究确定C1qbp是与衰老和不活动相关的肌肉损失的关键因素. 这种蛋白质通过干扰NFATc1/p300复合体来抑制肌肉生长,这表明C1qbp是对抗肌肉衰竭的潜在治疗标.
科学领域:
- 肌肉生理学 肌肉生理学
- 分子生物学分子生物学
- 衰老的研究研究.
背景情况:
- 肌肉损失 (sarcopenia) 是与衰老和减少体力活动相关的重大健康问题.
- 识别导致肌肉缩的新型分子机制对于开发有效干预措施至关重要.
研究的目的:
- 识别在老年和运动限制条件下导致肌肉损失的新型分子因素.
- 研究C1qbp在调节肌肉发育中的作用及其作为肌肉消耗治疗点的潜力.
主要方法:
- 从年轻,老年,炼和后肢不负荷的模型中对小鼠胃半腹肌的蛋白质组分析.
- 在体外研究评估C1qbp对肌体分化和NFATc1表达的影响.
- 分析C1qbp,NFATc1和同活性剂p300之间的相互作用.
主要成果:
- 在老年和后肢不负荷的小鼠肌肉中,C1qbp表达显著上调.
- 细胞外C1qbp通过抑制NFATc1/p300复合体来抑制肌体分化,从而降低了乙化希斯H3水平.
- 在骨肌肉中,C1qbp表达与NFATc1表达呈反向相关性.
结论:
- 细胞外C1qbp在抑制肌肉发育方面发挥了新的作用.
- C1qbp通过抑制NFATc1/p300复合体而起作用,NFATc1/p300复合体是肌肉分化的关键途径.
- C1qbp代表了一种有前途的治疗标,用于减轻与年龄相关的和不活动引起的肌肉损失.
相关概念视频
NF-κB-dependent Signaling Pathway
7.4K
The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
NF-κB-dependent Signaling Mechanism
The...
7.4K
Formation of Muscle Fibers from Myoblasts
4.9K
De novo myogenesis, or the formation of muscle fibers, begins during the early embryonic stages. The skeletal muscle is formed from somites– blocks of embryonic cell layers. The somites are further divided into dermatomes, myotomes, sclerotomes, and syndetomes. Among these, the myotomes give rise to muscle fibers.
Muscle progenitor cells (MPCs) are formed from the myotomes. MPCs express genes that encode the transcription factors Pax3 and Pax7. Along with Pax 3/7, other transcription...
Muscle progenitor cells (MPCs) are formed from the myotomes. MPCs express genes that encode the transcription factors Pax3 and Pax7. Along with Pax 3/7, other transcription...
4.9K
Anaphase Promoting Complex
2.8K
The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
2.8K
TGF - β Signaling Pathway
7.3K
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
7.3K
Regulation of Nuclear Protein Sorting
2.4K
Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
2.4K
Co-activators and Co-repressors
7.3K
Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
7.3K

